|
|
||
|
|
|
|
|
UniProt functional annotation for P9WPF1 | ||
|
|
|
|
|
|
||
| UniProt code: P9WPF1. |
|
||
| Organism: | |
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv). |
| Taxonomy: | |
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. |
|
||
|
||
| Function: | |
Involved in the biosynthesis of tri- and tetraketide alpha- pyrones. Pks18 catalyzes the extension of medium- and long-chain aliphatic acyl-CoA substrates by using malonyl-CoA as an extender molecule to synthesize polyketide products. {ECO:0000269|PubMed:12941968, ECO:0000269|PubMed:15286723, ECO:0000269|PubMed:15984864}. |
|
||
| Catalytic activity: | |
Reaction=2 H(+) + hexanoyl-CoA + 2 malonyl-CoA = 4-hydroxy-6- pentylpyran-2-one + 2 CO2 + 3 CoA; Xref=Rhea:RHEA:43452, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:62620, ChEBI:CHEBI:66958; Evidence={ECO:0000269|PubMed:12941968, ECO:0000269|PubMed:15286723}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43453; Evidence={ECO:0000269|PubMed:12941968, ECO:0000269|PubMed:15286723}; |
| Catalytic activity: | |
Reaction=2 H(+) + 2 malonyl-CoA + octanoyl-CoA = 4-hydroxy-6- heptylpyran-2-one + 2 CO2 + 3 CoA; Xref=Rhea:RHEA:44284, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:57386, ChEBI:CHEBI:84246; Evidence={ECO:0000269|PubMed:15286723}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:44285; Evidence={ECO:0000269|PubMed:15286723}; |
| Catalytic activity: | |
Reaction=dodecanoyl-CoA + 2 H(+) + 2 malonyl-CoA = 4-hydroxy-6- undecylpyran-2-one + 2 CO2 + 3 CoA; Xref=Rhea:RHEA:43460, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57375, ChEBI:CHEBI:57384, ChEBI:CHEBI:84149; Evidence={ECO:0000269|PubMed:12941968, ECO:0000269|PubMed:15286723, ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43461; Evidence={ECO:0000269|PubMed:12941968, ECO:0000269|PubMed:15286723, ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=dodecanoyl-CoA + 3 H(+) + 3 malonyl-CoA = 4-hydroxy-6-(2- oxotridecyl)pyran-2-one + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:43368, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57375, ChEBI:CHEBI:57384, ChEBI:CHEBI:84150; Evidence={ECO:0000269|PubMed:12941968, ECO:0000269|PubMed:15286723, ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43369; Evidence={ECO:0000269|PubMed:12941968, ECO:0000269|PubMed:15286723, ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=2 H(+) + hexadecanoyl-CoA + 2 malonyl-CoA = 4-hydroxy-6- pentadecylpyran-2-one + 2 CO2 + 3 CoA; Xref=Rhea:RHEA:44260, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:57384, ChEBI:CHEBI:84168; Evidence={ECO:0000269|PubMed:12941968, ECO:0000269|PubMed:15286723}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:44261; Evidence={ECO:0000269|PubMed:12941968, ECO:0000269|PubMed:15286723}; |
| Catalytic activity: | |
Reaction=3 H(+) + hexadecanoyl-CoA + 3 malonyl-CoA = 4-hydroxy-6-(2- oxoheptadecyl)pyran-2-one + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:44268, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:57384, ChEBI:CHEBI:84171; Evidence={ECO:0000269|PubMed:12941968, ECO:0000269|PubMed:15286723}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:44269; Evidence={ECO:0000269|PubMed:12941968, ECO:0000269|PubMed:15286723}; |
| Catalytic activity: | |
Reaction=2 H(+) + 2 malonyl-CoA + octadecanoyl-CoA = 4-hydroxy-6- heptadecylpyran-2-one + 2 CO2 + 3 CoA; Xref=Rhea:RHEA:44280, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:57394, ChEBI:CHEBI:84169; Evidence={ECO:0000269|PubMed:12941968, ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:44281; Evidence={ECO:0000269|PubMed:12941968, ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=3 H(+) + 3 malonyl-CoA + octadecanoyl-CoA = 4-hydroxy-6-(2- oxononadecyl)pyran-2-one + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:44272, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:57394, ChEBI:CHEBI:84172; Evidence={ECO:0000269|PubMed:12941968, ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:44273; Evidence={ECO:0000269|PubMed:12941968, ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=eicosanoyl-CoA + 2 H(+) + 2 malonyl-CoA = 4-hydroxy-6- nonadecylpyran-2-one + 2 CO2 + 3 CoA; Xref=Rhea:RHEA:44264, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57380, ChEBI:CHEBI:57384, ChEBI:CHEBI:84170; Evidence={ECO:0000269|PubMed:12941968}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:44265; Evidence={ECO:0000269|PubMed:12941968}; |
| Catalytic activity: | |
Reaction=eicosanoyl-CoA + 3 H(+) + 3 malonyl-CoA = 4-hydroxy-6-(2- oxohenicosyl)pyran-2-one + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:44276, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57380, ChEBI:CHEBI:57384, ChEBI:CHEBI:84173; Evidence={ECO:0000269|PubMed:12941968}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:44277; Evidence={ECO:0000269|PubMed:12941968}; |
| Catalytic activity: | |
Reaction=9-decenoyl-CoA + 2 H(+) + 2 malonyl-CoA = 4-hydroxy-6-(non-8- en-1-yl)-pyran-2-one + 2 CO2 + 3 CoA; Xref=Rhea:RHEA:45868, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:84214, ChEBI:CHEBI:85479; Evidence={ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45869; Evidence={ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=9-decenoyl-CoA + 3 H(+) + 3 malonyl-CoA = 4-hydroxy-6-(2- oxoundec-10-en-1-yl)pyran-2-one + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:45924, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:84214, ChEBI:CHEBI:85494; Evidence={ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45925; Evidence={ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=3-hydroxytetradecanoyl-CoA + 2 H(+) + 2 malonyl-CoA = 4- hydroxy-6-(2-hydroxytridecyl)-pyran-2-one + 2 CO2 + 3 CoA; Xref=Rhea:RHEA:45856, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:84198, ChEBI:CHEBI:85474; Evidence={ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45857; Evidence={ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=15-hydroxypentadecanoyl-CoA + 2 H(+) + 2 malonyl-CoA = 4- hydroxy-6-(14-hydroxytetradecyl)-pyran-2-one + 2 CO2 + 3 CoA; Xref=Rhea:RHEA:45872, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:84205, ChEBI:CHEBI:85481; Evidence={ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45873; Evidence={ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=15-hydroxypentadecanoyl-CoA + 3 H(+) + 3 malonyl-CoA = 4- hydroxy-6-(16-hydroxy-2-oxohexadecyl)pyran-2-one + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:45928, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:84205, ChEBI:CHEBI:85495; Evidence={ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45929; Evidence={ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=16-hydroxyhexadecanoyl-CoA + 2 H(+) + 2 malonyl-CoA = 4- hydroxy-6-(15-hydroxypentadecyl)-pyran-2-one + 2 CO2 + 3 CoA; Xref=Rhea:RHEA:45864, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:84207, ChEBI:CHEBI:85477; Evidence={ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45865; Evidence={ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=16-hydroxyhexadecanoyl-CoA + 3 H(+) + 3 malonyl-CoA = 4- hydroxy-6-(17-hydroxy-2-oxoheptadecyl)pyran-2-one + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:45920, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:84207, ChEBI:CHEBI:85492; Evidence={ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45921; Evidence={ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=12-hydroxyoctadecanoyl-CoA + 2 H(+) + 2 malonyl-CoA = 4- hydroxy-6-(11-hydroxyheptadecyl)-pyran-2-one + 2 CO2 + 3 CoA; Xref=Rhea:RHEA:45860, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:84202, ChEBI:CHEBI:85475; Evidence={ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45861; Evidence={ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=12-hydroxyoctadecanoyl-CoA + 3 H(+) + 3 malonyl-CoA = 4- hydroxy-6-(13-hydroxy-2-oxononadecyl)pyran-2-one + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:45916, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:84202, ChEBI:CHEBI:85490; Evidence={ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45917; Evidence={ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=2-methylhexadecanoyl-CoA + 2 H(+) + 2 malonyl-CoA = 6- (hexadecan-2-yl)-4-hydroxy-pyran-2-one + 2 CO2 + 3 CoA; Xref=Rhea:RHEA:45876, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:84182, ChEBI:CHEBI:85482; Evidence={ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45877; Evidence={ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=2-methylhexadecanoyl-CoA + 3 H(+) + 3 malonyl-CoA = 4-hydroxy- 6-(3-methyl-2-oxoheptadecyl)pyran-2-one + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:45932, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:84182, ChEBI:CHEBI:85496; Evidence={ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45933; Evidence={ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=3-methylundecanoyl-CoA + 2 H(+) + 2 malonyl-CoA = 4-hydroxy-6- (2-methyldecyl)-pyran-2-one + 2 CO2 + 3 CoA; Xref=Rhea:RHEA:45880, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:84183, ChEBI:CHEBI:85483; Evidence={ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45881; Evidence={ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=3-methylundecanoyl-CoA + 3 H(+) + 3 malonyl-CoA = 4-hydroxy-6- (4-methyl-2-oxododecyl)pyran-2-one + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:45936, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:84183, ChEBI:CHEBI:85497; Evidence={ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45937; Evidence={ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=12-methyltridecanoyl-CoA + 2 H(+) + 2 malonyl-CoA = 4-hydroxy- 6-(11-methyldodecyl)-pyran-2-one + 2 CO2 + 3 CoA; Xref=Rhea:RHEA:45888, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:84195, ChEBI:CHEBI:85485; Evidence={ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45889; Evidence={ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=12-methyltridecanoyl-CoA + 3 H(+) + 3 malonyl-CoA = 4-hydroxy- 6-(13-methyl-2-oxotetradecyl)pyran-2-one + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:45944, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:84195, ChEBI:CHEBI:85499; Evidence={ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45945; Evidence={ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=12-methyloctadecanoyl-CoA + 2 H(+) + 2 malonyl-CoA = 4- hydroxy-6-(11-methylheptadecyl)-pyran-2-one + 2 CO2 + 3 CoA; Xref=Rhea:RHEA:45884, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:84181, ChEBI:CHEBI:85484; Evidence={ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45885; Evidence={ECO:0000269|PubMed:15984864}; |
| Catalytic activity: | |
Reaction=12-methyloctadecanoyl-CoA + 3 H(+) + 3 malonyl-CoA = 4- hydroxy-6-(13-methyl-2-oxononadecyl)pyran-2-one + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:45940, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:84181, ChEBI:CHEBI:85498; Evidence={ECO:0000269|PubMed:15984864}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45941; Evidence={ECO:0000269|PubMed:15984864}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=4.2 uM for lauroyl-CoA (at 30 degrees Celsius and at pH 7.5) {ECO:0000269|PubMed:12941968}; KM=5.4 uM for palmitoyl-CoA (at 30 degrees Celsius and at pH 7.5) {ECO:0000269|PubMed:12941968}; KM=6.1 uM for arachidoyl-CoA (at 30 degrees Celsius and at pH 7.5) {ECO:0000269|PubMed:12941968}; KM=19.2 uM for hexanoyl-CoA (at 30 degrees Celsius and at pH 7.5) {ECO:0000269|PubMed:12941968}; KM=49.2 uM for acetyl-CoA (at 30 degrees Celsius and at pH 7.5) {ECO:0000269|PubMed:12941968}; KM=58.4 uM for malonyl-CoA (at 30 degrees Celsius and at pH 7.5) {ECO:0000269|PubMed:12941968}; |
| Pathway: | |
Lipid metabolism; fatty acid biosynthesis. {ECO:0000305}. |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:15286723}. |
| Similarity: | |
Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.