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PDBsum entry 1spf
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Lipoprotein(surface film)
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PDB id
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1spf
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References listed in PDB file
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Key reference
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Title
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The nmr structure of the pulmonary surfactant-Associated polypeptide sp-C in an apolar solvent contains a valyl-Rich alpha-Helix.
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Authors
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J.Johansson,
T.Szyperski,
T.Curstedt,
K.Wüthrich.
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Ref.
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Biochemistry, 1994,
33,
6015-6023.
[DOI no: ]
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PubMed id
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Abstract
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The nuclear magnetic resonance (NMR) structure of the pulmonary
surfactant-associated lipoplypeptide C (SP-C) was determined in a mixed solvent
of C2H3Cl/C2H3OH/ 1 M HCl 32:64:5 (v/v). Sequence-specific 1H NMR assignments
and the collection of conformational constraints were achieved with
two-dimensional 1H NMR, and the structure was calculated with the distance
geometry program DIANA. The root mean square deviations for the well-defined
polypeptide segment of residues 9-34 calculated for the 20 best energy-minimized
DIANA conformers relative to their mean are 0.5 and 1.3 A for the polypeptide
backbone atoms N, C alpha, and C', and for all heavy atoms, respectively. The
35-residue polypeptide chain of SP-C forms an alpha-helix between positions 9
and 34, which includes two segments of seven and four consecutive valyls that
are separated by a single leucyl residue. The N-terminal hexapeptide segment,
which includes two palmitoylcysteinyls, is flexibly disordered. The length of
the alpha-helix is about 37 A, and the helical segment of residues 13-28, which
contains exclusively aliphatic residues with branched side chains, is 23-A long
and about 10 A in diameter. The alpha-helix is outstandingly regular, with
virtually identical chi 1 angles for all valyl residues. The observation of a
helical structure of SP-C was rather unexpected, considering that Val is
generally underrepresented in alpha-helices, and it provides intriguing novel
insights into the structural basis of SP-C functions as well as into general
structural aspects of protein-lipid interactions in biological membranes.
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Secondary reference #1
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Title
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Size and structure of the hydrophobic low molecular weight surfactant-Associated polypeptide.
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Authors
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J.Johansson,
T.Curstedt,
B.Robertson,
H.Jörnvall.
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Ref.
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Biochemistry, 1988,
27,
3544-3547.
[DOI no: ]
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PubMed id
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