PDBsum entry 1slt

Lectin

PDB id: 1slt

Contents

Protein chains

133 a.a. *

Ligands

NDG-GAL ×2

Metals

_CL

Waters ×154

* Residue conservation analysis

References listed in PDB file

Key reference

• Title: Structure of s-Lectin, A developmentally regulated vertebrate beta-Galactoside-Binding protein.
• Authors: D.I.Liao, G.Kapadia, H.Ahmed, G.R.Vasta, O.Herzberg.
• Ref.: Proc Natl Acad Sci U S A, 1994, 91, 1428-1432. [DOI no: 10.1073/pnas.91.4.1428]
• PubMed id: 8108426

Abstract

The crystal structure of a 14-kDa bovine spleen S-lectin complexed with the disaccharide N-acetyllactosamine at 1.9-A resolution reveals a surprising structural relationship to legume lectins, despite the lack of sequence homology. Two monomers associate to form an extended beta-sandwich, each with the same jelly roll topology typical of legume lectins but with dramatically trimmed loops and with different dimer association. Each monomer binds one N-acetyllactosamine molecule in a topologically and spatially different site than that of legume lectins. The carbohydrate-binding site provides an unprecedented paradigm for carbohydrate binding, with a unique network of salt bridges. The specificity for beta-galactose arises from intricate interactions that constrain the position of the O4 atom.