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PDBsum entry 1sce
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Cell cycle regulatory protein
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PDB id
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1sce
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Contents |
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97 a.a.
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111 a.a.
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103 a.a.
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Crystal structure of the cell cycle-Regulatory protein suc1 reveals a beta-Hinge conformational switch.
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Authors
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Y.Bourne,
A.S.Arvai,
S.L.Bernstein,
M.H.Watson,
S.I.Reed,
J.E.Endicott,
M.E.Noble,
L.N.Johnson,
J.A.Tainer.
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Ref.
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Proc Natl Acad Sci U S A, 1995,
92,
10232-10236.
[DOI no: ]
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PubMed id
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Abstract
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The Schizosaccharomyces pombe cell cycle-regulatory protein suc1, named as the
suppressor of cdc2 temperature-sensitive mutations, is essential for cell cycle
progression. To understand suc1 structure-function relationships and to help
resolve conflicting interpretations of suc1 function based on genetic studies of
suc1 and its functional homologs in both lower and higher eukaryotes, we have
determined the crystal structure of the beta-interchanged suc1 dimer. Each
domain consists of three alpha-helices and a four-stranded beta-sheet, completed
by the interchange of terminal beta-strands between the two subunits. This
beta-interchanged suc1 dimer, when compared with the beta-hairpin single-domain
folds of suc1, reveals a beta-hinge motif formed by the conserved amino acid
sequence HVPEPH. This beta-hinge mediates the subunit conformation and assembly
of suc1: closing produces the intrasubunit beta-hairpin and single-domain fold,
whereas opening leads to the intersubunit beta-strand interchange and
interlocked dimer assembly reported here. This conformational switch markedly
changes the surface accessibility of sequence-conserved residues available for
recognition of cyclin-dependent kinase, suggesting a structural mechanism for
beta-hinge-mediated regulation of suc1 biological function. Thus, suc1 belongs
to the family of domain-swapping proteins, consisting of intertwined and dimeric
protein structures in which the dual assembly modes regulate their function.
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Secondary reference #1
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Title
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The crystal structure of p13suc1, A p34cdc2-Interacting cell cycle control protein.
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Authors
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J.A.Endicott,
M.E.Noble,
E.F.Garman,
N.Brown,
B.Rasmussen,
P.Nurse,
L.N.Johnson.
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Ref.
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Embo J, 1995,
14,
1004-1014.
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PubMed id
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