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PDBsum entry 1s9h
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Crystal structure of the sf3 helicase from adeno-Associated virus type 2.
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Authors
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J.A.James,
C.R.Escalante,
M.Yoon-Robarts,
T.A.Edwards,
R.M.Linden,
A.K.Aggarwal.
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Ref.
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Structure, 2003,
11,
1025-1035.
[DOI no: ]
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PubMed id
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Abstract
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We report here the crystal structure of an SF3 DNA helicase, Rep40, from
adeno-associated virus 2 (AAV2). We show that AAV2 Rep40 is structurally more
similar to the AAA(+) class of cellular proteins than to DNA helicases from
other superfamilies. The structure delineates the expected Walker A and B
motifs, but also reveals an unexpected "arginine finger" that directly
implies the requirement of Rep40 oligomerization for ATP hydrolysis and helicase
activity. Further, the Rep40 AAA(+) domain is novel in that it is unimodular as
opposed to bimodular. Altogether, the structural connection to AAA(+) proteins
defines the general architecture of SF3 DNA helicases, a family that includes
simian virus 40 (SV40) T antigen, as well as provides a conceptual framework for
understanding the role of Rep proteins during AAV DNA replication, packaging,
and site-specific integration.
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Figure 2.
Figure 2. Structure and Topology of AAV2 Rep40(A) Ribbon
representation of the AAV2 Rep40 structure. The N-terminal
a-helical domain is shown in blue. The C-terminal a/b (vAAA^+)
domain is colored gold. The a helices (a1-a11) are labeled
sequentially from the N to the C terminus. b strands are labeled
according to their belonging to the central b core (b1-b5) or to
the b hairpins (ba-bd).(B) Topology diagrams of representative
members of helicase and AAA^+ superfamilies. Gold, NTPase cores;
green, extra functional domains; blue, Rep40 N-terminal domain.
NSF-D2, D2 domain of N-ethylmaleimide-sensitive fusion protein;
PcrA, DNA helicase from B. stereothermophilus; HCV, hepatitis C
virus nonstructural protein 3 protease-helicase; T7gp4,
bacteriophage T7 gene 4 primase-helicase.
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The above figure is
reprinted
by permission from Cell Press:
Structure
(2003,
11,
1025-1035)
copyright 2003.
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