UniProt functional annotation for P32324



	UniProt functional annotation for P32324

UniProt code: P32324.

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).

Taxonomy: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.

Function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. {ECO:0000269|PubMed:14976550}.

Activity regulation: Inhibited by fusidic acid and sordarin, which prevent the release of eEF2 from the ribosome after the translocation step. While fusidic acid acts on all eukaryotic eEF2, sordarin specifically binds and inhibits only selected fungal eEF2. Inhibited by diphtheria toxin and Pseudomonas aeruginosa exotoxin A (ETA), which ADP-ribosylate the diphthamide residue.

Biophysicochemical properties: Kinetic parameters: KM=0.010 mM for GTP;

Pathway: Protein biosynthesis; polypeptide chain elongation.

Subunit: Binds to 80S ribosomes. Interacts directly with the 40S ribosomal subunit protein RPL9A, the 60S ribosomal subunit proteins RPL12A and RPS23A, as well as the 18S rRNA and the 25S rRNA. Interacts with RPL0. {ECO:0000269|PubMed:12410829, ECO:0000269|PubMed:12692531, ECO:0000269|PubMed:14976550, ECO:0000269|PubMed:15316019}.

Subcellular location: Cytoplasm {ECO:0000269|PubMed:14562095}.

Miscellaneous: Present with 160782 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.

Miscellaneous: There are 2 genes for eEF2 in yeast.

Similarity: Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily. {ECO:0000255|PROSITE-ProRule:PRU01059}.

Annotations taken from UniProtKB at the EBI.


Organism:		Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Taxonomy:		Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.



Function:		Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. {ECO:0000269\|PubMed:14976550}.


Activity regulation:		Inhibited by fusidic acid and sordarin, which prevent the release of eEF2 from the ribosome after the translocation step. While fusidic acid acts on all eukaryotic eEF2, sordarin specifically binds and inhibits only selected fungal eEF2. Inhibited by diphtheria toxin and Pseudomonas aeruginosa exotoxin A (ETA), which ADP-ribosylate the diphthamide residue.
Biophysicochemical properties:		Kinetic parameters: KM=0.010 mM for GTP;
Pathway:		Protein biosynthesis; polypeptide chain elongation.
Subunit:		Binds to 80S ribosomes. Interacts directly with the 40S ribosomal subunit protein RPL9A, the 60S ribosomal subunit proteins RPL12A and RPS23A, as well as the 18S rRNA and the 25S rRNA. Interacts with RPL0. {ECO:0000269\|PubMed:12410829, ECO:0000269\|PubMed:12692531, ECO:0000269\|PubMed:14976550, ECO:0000269\|PubMed:15316019}.
Subcellular location:		Cytoplasm {ECO:0000269\|PubMed:14562095}.
Miscellaneous:		Present with 160782 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Miscellaneous:		There are 2 genes for eEF2 in yeast.
Similarity:		Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily. {ECO:0000255\|PROSITE-ProRule:PRU01059}.