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UniProt functional annotation for Q9FCA6 | ||
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| UniProt code: Q9FCA6. |
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| Organism: | |
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145). |
| Taxonomy: | |
Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces albidoflavus group. |
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| Function: | |
Catalyzes oxidative C-C coupling reaction to polymerize flaviolin and form highly conjugated pigments which protect the soil bacterium from deleterious effects of UV irradiation (three isomers of biflaviolin and one triflaviolin). {ECO:0000269|PubMed:15659395, ECO:0000269|PubMed:16239228}. |
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| Catalytic activity: | |
Reaction=2 flaviolin + H(+) + O2 + 2 reduced [2Fe-2S]-[ferredoxin] = 3,3'-biflaviolin + 2 H2O + 2 oxidized [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:26031, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:58696, ChEBI:CHEBI:77877; EC=1.14.19.69; Evidence={ECO:0000269|PubMed:15659395, ECO:0000269|PubMed:16239228, ECO:0000269|PubMed:22203090}; |
| Catalytic activity: | |
Reaction=2 flaviolin + H(+) + O2 + 2 reduced [2Fe-2S]-[ferredoxin] = 3,8'-biflaviolin + 2 H2O + 2 oxidized [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:26035, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:58696, ChEBI:CHEBI:77840; EC=1.14.19.69; Evidence={ECO:0000269|PubMed:15659395, ECO:0000269|PubMed:16239228, ECO:0000269|PubMed:22203090}; |
| Cofactor: | |
Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000269|PubMed:15659395, ECO:0000269|PubMed:16239228, ECO:0000269|PubMed:22203090}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=7.3 uM for flaviolin (at pH 8.2 and 37 degrees Celsius) {ECO:0000269|PubMed:22203090}; Note=kcat is 1.4 min(-1) with flaviolin as substrate (at pH 7.5 and 37 degrees Celsius). kcat is 0.02 min(-1) with 2-hydroxy-1,4- naphthoquinone as substrate (at pH 7.5 and 25 degrees Celsius). {ECO:0000269|PubMed:15659395, ECO:0000269|PubMed:16239228}; |
| Pathway: | |
Pigment biosynthesis. {ECO:0000303|PubMed:15659395}. |
| Miscellaneous: | |
The structural studies suggest catalysis likely occurs through proton relay via a 'proton wire' formed by water molecules in the active site. {ECO:0000269|PubMed:16239228, ECO:0000269|PubMed:22203090}. |
| Similarity: | |
Belongs to the cytochrome P450 family. {ECO:0000255|RuleBase:RU000461}. |
Annotations taken from UniProtKB at the EBI.