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PDBsum entry 1s03
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Transcription/RNA
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PDB id
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1s03
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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The structure of a ribosomal protein s8/spc operon mRNA complex.
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Authors
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H.J.Merianos,
J.Wang,
P.B.Moore.
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Ref.
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Rna, 2004,
10,
954-964.
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PubMed id
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Abstract
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In bacteria, translation of all the ribosomal protein cistrons in the spc operon
mRNA is repressed by the binding of the product of one of them, S8, to an
internal sequence at the 5' end of the L5 cistron. The way in which the first
two genes of the spc operon are regulated, retroregulation, is mechanistically
distinct from translational repression by S8 of the genes from L5 onward. A 2.8
A resolution crystal structure has been obtained of Escherichia coli S8 bound to
this site. Despite sequence differences, the structure of this complex is almost
identical to that of the S8/helix 21 complex seen in the small ribosomal
subunit, consistent with the hypothesis that autogenous regulation of ribosomal
protein synthesis results from conformational similarities between mRNAs and
rRNAs. S8 binding must repress the translation of its own mRNA by inhibiting the
formation of a ribosomal initiation complex at the start of the L5 cistron.
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