PDBsum entry 1rtq

Hydrolase

PDB id: 1rtq

Contents

Protein chain

291 a.a. *

Ligands

SCN ×2

Metals

_NA ×3

_ZN ×2

Waters ×324

* Residue conservation analysis

References listed in PDB file

Key reference

• Title: The high-Resolution structures of the neutral and the low ph crystals of aminopeptidase from aeromonas proteolytica.
• Authors: W.Desmarais, D.L.Bienvenue, K.P.Bzymek, G.A.Petsko, D.Ringe, R.C.Holz.
• Ref.: J Biol Inorg Chem, 2006, 11, 398-408.
• PubMed id: 16596389

Abstract

The aminopeptidase from Aeromonas proteolytica (AAP) contains two zinc ions in the active site and catalyzes the degradation of peptides. Herein we report the crystal structures of AAP at 0.95-A resolution at neutral pH and at 1.24-A resolution at low pH. The combination of these structures allowed the precise modeling of atomic positions, the identification of the metal bridging oxygen species, and insight into the physical properties of the metal ions. On the basis of these structures, a new putative catalytic mechanism is proposed for AAP that is likely relevant to all binuclear metalloproteases.