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PDBsum entry 1rof
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Electron transport
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PDB id
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1rof
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References listed in PDB file
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Key reference
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Title
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An nmr-Derived model for the solution structure of oxidized thermotoga maritima 1[fe4-S4] ferredoxin.
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Authors
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H.Sticht,
G.Wildegger,
D.Bentrop,
B.Darimont,
R.Sterner,
P.Rösch.
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Ref.
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Eur J Biochem, 1996,
237,
726-735.
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PubMed id
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Abstract
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The solution structure of the 60-residue 1[Fe4-S4] ferredoxin from the
hyperthermophilic bacterium Thermotoga maritima was determined based on 683
distance and 35 dihedral angle restraints that were obtained from NMR data. In
addition, data known from crystallographic studies of ferredoxins was used for
modeling of the iron-sulfur cluster and its environment. The protein shows a
globular fold very similar to the fold of the related 1[Fe4-S4] ferredoxins from
Desulfovibrio gigas and Desulfovibrio africanus, and elements of regular
secondary structure similar to those in other ferredoxins were found in the T.
maritima protein. In particular, the T. maritima protein displayed a beta-sheet
structure made up of strands located at the very NH(2) and COOH termini of the
protein, and an internal alpha-helix. The internal beta-sheet observed in the D.
gigas and D. africanus ferredoxins could not be confirmed in T. maritima
ferredoxin and is thus suggested to be only weakly present or even absent in
this protein. This result suggests that thermostability in ferredoxins is not
necessarily correlated with the content of stable elements of regular secondary
structure.
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