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PDBsum entry 1rmf
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Immunoglobulin
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PDB id
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1rmf
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Structure of a monoclonal anti-Icam-1 antibody r6.5 FAB fragment at 2.8 a resolution.
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Authors
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M.J.Jedrzejas,
J.Miglietta,
J.A.Griffin,
M.Luo.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 1995,
51,
380-385.
[DOI no: ]
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PubMed id
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Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
percentage match of
89%.
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Abstract
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The specific binding of the monoclonal murine anti-intercellular adhesion
molecule-1 (anti-ICAM-1) antibody, R6.5, inhibits the attachment of neutrophils
to endothelium and prevents the attachment of major group human rhinovirus (HRV)
to ICAM-1. This binding interferes with the host immune system and, as a result,
the R6.5 antibody has been developed as a therapeutic anti-inflammatory and
perhaps anti-HRV agent. The variable-region amino-acid sequence of R6.5 was
determined from the anti-ICAM-1 cDNA. The crystallization conditions of the Fab
fragment of R6.5 were established and the three-dimensional structure was
determined by X-ray crystallography. The crystal space group is orthorhombic
P2(1)2(1)2(1), a = 40.36, b = 137.76, c = 91.32 A, and the highest resolution of
recorded reflections is 2.7 A. The molecular-replacement method using known Fab
structures was employed to solve the R6.5 Fab structure. The final R-factor is
18.8% for a total of 3320 non-H protein atoms, 39 water molecules and 10 606
unique reflections. The protein exhibits the typical immunoglobulin fold. The
surface contour of the antigen-combining site of the R6.5 antibody has a wide
groove which resembles more the structure of an anti-polypeptide antibody than
the structure of an anti-protein antibody.
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Figure 2.
Fig. 2. A photograph of R6.5 Fab crystals grown in a hanging drop. The
rystallization conditions re described in Materials and methods.
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Figure 7.
Fig. 7. The surface contour of R6.5
CDRs. The color code for CDRs
is, CDRL1, magenta, L2, dark
blue, L3, light blue, CDRH1
red, H2, green and H3, yellow.
The drawing was made using
the RIBBONS program (Caron,
1987).
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The above figures are
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(1995,
51,
380-385)
copyright 1995.
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