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UniProt functional annotation for Q8SR66 | ||
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| UniProt code: Q8SR66. |
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| Organism: | |
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite). |
| Taxonomy: | |
Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Unikaryonidae; Encephalitozoon. |
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| Function: | |
mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC. {ECO:0000269|PubMed:15760890}. |
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| Catalytic activity: | |
Reaction=a 5'-end (5'-triphosphoguanosine)-(ribonucleoside) in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'- triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L- homocysteine; Xref=Rhea:RHEA:67008, Rhea:RHEA-COMP:17166, Rhea:RHEA- COMP:17167, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:156461, ChEBI:CHEBI:167617; EC=2.1.1.56; Evidence={ECO:0000250|UniProtKB:O43148, ECO:0000255|PROSITE- ProRule:PRU00895}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=1 mM for GTP {ECO:0000269|PubMed:15760890}; KM=25 uM for S-adenosyl-L-methionine {ECO:0000269|PubMed:15760890}; KM=0.1 mM for cap dinucleotide GpppA {ECO:0000269|PubMed:15760890}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:15760890, ECO:0000269|PubMed:16971388}. |
| Subcellular location: | |
Nucleus {ECO:0000250}. |
| Similarity: | |
Belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family. {ECO:0000255|PROSITE- ProRule:PRU00895}. |
Annotations taken from UniProtKB at the EBI.