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PDBsum entry 1rg0
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Cell adhesion
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PDB id
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1rg0
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References listed in PDB file
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Key reference
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Title
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Crystallographic analysis of the pseudomonas aeruginosa strain k122-4 monomeric pilin reveals a conserved receptor-Binding architecture.
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Authors
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G.F.Audette,
R.T.Irvin,
B.Hazes.
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Ref.
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Biochemistry, 2004,
43,
11427-11435.
[DOI no: ]
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PubMed id
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Abstract
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Adherence of pathogens to host cells is critical for the initiation of infection
and is thus an attractive target for anti-infective therapeutics and vaccines.
In the opportunistic human pathogen Pseudomonas aeruginosa, host-cell adherence
is achieved predominantly by type IV pili. Analysis of several clinical strains
of P. aeruginosa reveals poor sequence conservation between pilin genes,
including the residues in the receptor-binding site. Interestingly, the
receptor-binding sites appear to retain a conserved surface epitope because all
Pseudomonas type IV pili recognize the same receptor on the host cell and
cross-reactive antibodies specific for the receptor-binding site exist. Here, we
present the crystallographic analysis of two crystal forms of truncated pilin
from P. aeruginosa strain K122-4 (DeltaK122-4) at 1.54 and 1.8 A resolution,
respectively. The DeltaK122-4 structure is compared to other
crystallographically determined type IV pilin structures and an NMR structure of
DeltaK122-4 pilin. A comparison with the structure of the highly divergent P.
aeruginosa strain K (DeltaPAK) pilin indicates that the receptor-binding loop in
both pilins forms a shallow depression with a surface that is formed by
main-chain atoms. Conservation of this putative binding site is independent of
the sequence as long as the main-chain conformation is conserved and could
therefore explain the shared receptor specificity and antibody cross reactivity
of highly divergent Pseudomonas type IV pilins.
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Secondary reference #1
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Title
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Purification, Crystallization and preliminary diffraction studies of the pseudomonas aeruginosa strain k122-4 monomeric pilin.
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Authors
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G.F.Audette,
R.T.Irvin,
B.Hazes.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 2003,
59,
1665-1667.
[DOI no: ]
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PubMed id
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Figure 1.
Figure 1 A crystal of the monomeric K122-4 pilin. Typical
dimensions are 0.25 × 0.1 × 0.05 mm.
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The above figure is
reproduced from the cited reference
with permission from the IUCr
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