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UniProt functional annotation for O67082 | ||
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| UniProt code: O67082. |
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| Organism: | |
Aquifex aeolicus (strain VF5). |
| Taxonomy: | |
Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex. |
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| Function: | |
Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Probably processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism. {ECO:0000269|PubMed:16439209, ECO:0000269|PubMed:21138964}. |
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| Catalytic activity: | |
Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.3; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:11738048, ECO:0000269|PubMed:15016361, ECO:0000269|PubMed:16439209, ECO:0000269|PubMed:18047582, ECO:0000269|PubMed:21138964}; Note=Binds 2 Mg(2+) per subunit. Mn(2+) also supports catalytic activity. {ECO:0000269|PubMed:11738048, ECO:0000269|PubMed:15016361, ECO:0000269|PubMed:16439209, ECO:0000269|PubMed:18047582, ECO:0000269|PubMed:21138964}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=98 nM for 16S-u-hp RNA {ECO:0000269|PubMed:21138964}; pH dependence: Optimum pH is 8-9. {ECO:0000269|PubMed:21138964}; Temperature dependence: Optimum temperature is 70-85 degrees Celsius. {ECO:0000269|PubMed:21138964}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:11738048, ECO:0000269|PubMed:15016361, ECO:0000269|PubMed:16216575, ECO:0000269|PubMed:16439209, ECO:0000269|PubMed:18047582}. |
| Subcellular location: | |
Cytoplasm {ECO:0000250}. |
| Similarity: | |
Belongs to the ribonuclease III family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.