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PDBsum entry 1r1c
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Electron transport
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PDB id
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1r1c
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Spectroscopy and reactivity of a photogenerated tryptophan radical in a structurally defined protein environment.
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Authors
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J.E.Miller,
C.Grădinaru,
B.R.Crane,
A.J.Di bilio,
W.A.Wehbi,
S.Un,
J.R.Winkler,
H.B.Gray.
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Ref.
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J Am Chem Soc, 2003,
125,
14220-14221.
[DOI no: ]
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PubMed id
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Abstract
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Near-UV irradiation of structurally characterized
[Re(I)(CO)3(1,10-phenanthroline)(Q107H)](W48F/Y72F/H83Q/Y108W)AzM(II) [Az =
Pseudomonas aeruginosa azurin, M = Cu, Zn]/[Co(NH3)5Cl]Cl2 produces a tryptophan
radical (W108*) with unprecedented kinetic stability. After rapid formation (k =
2.8 x 106 s-1), the radical persists for more than 5 h at room temperature in
the folded ReAzM(II) structure. The absorption spectrum of ReAz(W108*)M(II)
exhibits maxima at 512 and 536 nm. Oxidation of K4[Mo(CN)8] by ReAz(W108*)Zn(II)
places the W108*/W108 reduction potential in the protein above 0.8 V vs NHE.
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