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UniProt functional annotation for Q04432 | ||
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| UniProt code: Q04432. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
Catalyzes the conversion of methylglyoxal (MG) to D-lactate in a single glutathione (GSH)-independent step. May play a role in detoxifying endogenously produced glyoxals (PubMed:24302734). Involved in protection against reactive oxygen species (ROS) (PubMed:17395014). Important for viability in stationary phase. May negatively regulate TORC1 in response to nutrient limitation (PubMed:24706893). {ECO:0000269|PubMed:17395014, ECO:0000269|PubMed:24302734, ECO:0000269|PubMed:24706893}. |
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| Catalytic activity: | |
Reaction=H2O + methylglyoxal = (R)-lactate + H(+); Xref=Rhea:RHEA:27754, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16004, ChEBI:CHEBI:17158; EC=4.2.1.130; Evidence={ECO:0000269|PubMed:24302734, ECO:0000269|PubMed:24758716}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=1.5 mM for methylglyoxal {ECO:0000269|PubMed:24758716}; Note=kcat is 75.0 min(-1) with methylglyoxal as substrate. {ECO:0000269|PubMed:24758716}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:14745011, ECO:0000269|PubMed:15130476}. |
| Subcellular location: | |
Cytoplasm, P-body {ECO:0000269|PubMed:24706893}. Note=Present in processing bodies (P-bodies) and stress granule (SG) foci upon glucose starvation and heat shock. {ECO:0000269|PubMed:24706893}. |
| Induction: | |
Up-regulated 10- to 30-fold during entry into stationary phase, by hydrogen peroxide or diamide stress, by heat stress, and by growth in the presence of the proline analog azetidine-2-carboxylic acid. {ECO:0000269|PubMed:17395014, ECO:0000269|PubMed:24706893}. |
| Ptm: | |
Cys-138 is easily oxidized to sulfinic acid. {ECO:0000269|PubMed:14745011}. |
| Disruption phenotype: | |
Results in higher sensitivity to oxidative stress, reduced thermotolerance, accumulation of higher levels of reactive oxygen species, and reduced chronological life span. {ECO:0000269|PubMed:17395014, ECO:0000269|PubMed:24706893}. |
| Miscellaneous: | |
No protease activity could be detected. {ECO:0000269|PubMed:12855764}. |
| Miscellaneous: | |
Present with 358 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the peptidase C56 family. HSP31-like subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.