|
|
||
|
|
|
|
|
UniProt functional annotation for P0AF93 | ||
|
|
|
|
|
|
||
| UniProt code: P0AF93. |
|
||
| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
|
||
|
||
| Function: | |
Accelerates the release of ammonia from reactive enamine/imine intermediates of the PLP-dependent threonine dehydratase (IlvA) in the low water environment of the cell. It catalyzes the deamination of enamine/imine intermediates to yield 2-ketobutyrate and ammonia. It is required for the detoxification of reactive intermediates of IlvA due to their highly nucleophilic abilities. Involved in the isoleucine biosynthesis (By similarity). {ECO:0000250}. |
|
||
| Catalytic activity: | |
Reaction=2-iminobutanoate + H2O = 2-oxobutanoate + NH4(+); Xref=Rhea:RHEA:39975, ChEBI:CHEBI:15377, ChEBI:CHEBI:16763, ChEBI:CHEBI:28938, ChEBI:CHEBI:76545; EC=3.5.99.10; |
| Catalytic activity: | |
Reaction=2-iminopropanoate + H2O = NH4(+) + pyruvate; Xref=Rhea:RHEA:40671, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:44400; EC=3.5.99.10; |
| Pathway: | |
Amino-acid biosynthesis; L-isoleucine biosynthesis; 2- oxobutanoate from L-threonine. |
| Subunit: | |
Homotrimer. {ECO:0000269|PubMed:10595546}. |
| Subcellular location: | |
Cytoplasm {ECO:0000305}. |
| Similarity: | |
Belongs to the RutC family. {ECO:0000305}. |
| Sequence caution: | |
Sequence=AAA97140.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.