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UniProt functional annotation for P80561 | ||
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| UniProt code: P80561. |
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| Organism: | |
Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350). |
| Taxonomy: | |
Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae; Streptomyces. |
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| Function: | |
An exopeptidase specific for larger hydrophobic amino acids (especially leucine), no cleavage occurs if the next residue is proline (PubMed:8444149). {ECO:0000269|PubMed:2503378, ECO:0000269|PubMed:8444149, ECO:0000269|PubMed:8665903}. |
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| Catalytic activity: | |
Reaction=Release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues.; EC=3.4.11.24; Evidence={ECO:0000269|PubMed:8444149}; |
| Cofactor: | |
Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000269|PubMed:15388919, ECO:0000269|PubMed:9048953}; Note=Binds 1 Ca(2+) per subunit. {ECO:0000269|PubMed:15388919, ECO:0000269|PubMed:9048953}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:15388919, ECO:0000269|PubMed:8444149, ECO:0000269|PubMed:9048953}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:8444149}; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Evidence={ECO:0000269|PubMed:8444149}; Note=Binds 2 Zn(2+) ions per subunit. Can be replaced by Mn(2+) or Co(2+) which results in altered specificities (PubMed:8444149). {ECO:0000269|PubMed:15388919, ECO:0000269|PubMed:8444149, ECO:0000269|PubMed:9048953}; |
| Activity regulation: | |
Calcium activates the enzyme, inhibited by 1,10- phenanthroline, EDTA and EGTA (PubMed:2503378). End-product inhibited by L-amino acids (PubMed:15388919). Non-competitively inhibited by NaF and NaH(2)PO(4) (PubMed:17608735). {ECO:0000269|PubMed:15388919, ECO:0000269|PubMed:17608735, ECO:0000269|PubMed:2503378, ECO:0000269|PubMed:8444149}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.59 mM for Leu-NH-Np (with zinc as cofactor) {ECO:0000269|PubMed:8444149}; KM=0.13 mM for Leu-NH-Np (with manganese as cofactor) {ECO:0000269|PubMed:8444149}; KM=0.046 mM for Leu-NH-Np (with cobalt as cofactor) {ECO:0000269|PubMed:8444149}; KM=4.1 mM for Ala-NH-Np (with zinc as cofactor) {ECO:0000269|PubMed:8444149}; KM=1.74 mM for Ala-NH-Np (with cobalt as cofactor) {ECO:0000269|PubMed:8444149}; KM=0.57 mM for Leu-pNA (with zinc as cofactor) {ECO:0000269|PubMed:15280041}; Note=kcat is 390 s(-1) with Leu-pNA. {ECO:0000269|PubMed:15280041}; Temperature dependence: Stable after heating at 69 degrees Celsius for 5 hours. {ECO:0000269|PubMed:2503378}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:2503378, ECO:0000269|PubMed:9048953}. |
| Subcellular location: | |
Secreted {ECO:0000269|PubMed:2503378, ECO:0000269|PubMed:8665903}. |
| Mass spectrometry: | |
Mass=29728; Mass_error=1.0; Method=Electrospray; Evidence={ECO:0000269|PubMed:8665903}; |
| Biotechnology: | |
Sold by several companies as an extracellular extract called Pronase. {ECO:0000305|PubMed:2503378, ECO:0000305|PubMed:8444149, ECO:0000305|PubMed:8665903}. |
| Miscellaneous: | |
Most experiments use protein purified from a commercial source rather than protein that has been overexpressed. {ECO:0000305}. |
| Similarity: | |
Belongs to the peptidase M28 family. M28A subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.