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PDBsum entry 1ql5
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References listed in PDB file
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Key reference
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Title
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Solution structure of the DNA decamer duplex containing a 3'-T x t basepair of the cis-Syn cyclobutane pyrimidine dimer: implication for the mutagenic property of the cis-Syn dimer.
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Authors
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J.H.Lee,
Y.J.Choi,
B.S.Choi.
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Ref.
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Nucleic Acids Res, 2000,
28,
1794-1801.
[DOI no: ]
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PubMed id
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Abstract
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The cis - syn dimer is the major DNA photoproduct produced by UV irradiation. In
order to determine the origin of the mutagenic property of the cis - syn dimer,
we used NMR restraints and molecular dynamics to determine the solution
structure of a DNA decamer duplex containing a wobble pair between the 3'-T of
the cis - syn dimer and the opposite T residue (CS/TA duplex). The solution
structure of the CS/TA duplex revealed that the 3'-T x T base pair of the cis -
syn dimer had base pair geometry that was significantly different from the
canonical Watson-Crick base pair and caused destabilization and conformational
distortion of its 3'-region. However, a 3'-T x A base pair at the cis - syn
dimer within this related DNA decamer maintains the normal Watson-Crick base
pair geometry and causes little distortion in the conformation of its 3'-side.
Our results show that in spite of its stable hydrogen bonding, the insertion of
a T residue opposite the 3'-T of the cis - syn dimer is inhibited by structural
distortion caused by the 3'-T x T base pair. This may explain why the frequency
of the 3'-T-->A transversion, which is the major mutation produced by the cis
- syn dimer, is only 4%.
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Headers
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