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UniProt functional annotation for P69902 | ||
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| UniProt code: P69902. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Involved in the catabolism of oxalate and in the adapatation to low pH via the induction of the oxalate-dependent acid tolerance response (ATR). Catalyzes the transfer of the CoA moiety from formyl- CoA to oxalate. It can also use succinate as an acceptor. {ECO:0000269|PubMed:12844490, ECO:0000269|PubMed:18245280, ECO:0000269|PubMed:23335415}. |
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| Catalytic activity: | |
Reaction=formyl-CoA + oxalate = formate + oxalyl-CoA; Xref=Rhea:RHEA:16545, ChEBI:CHEBI:15740, ChEBI:CHEBI:30623, ChEBI:CHEBI:57376, ChEBI:CHEBI:57388; EC=2.8.3.16; Evidence={ECO:0000255|HAMAP-Rule:MF_00742, ECO:0000269|PubMed:18245280}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=180 uM for formyl-CoA (with succinate as acceptor at pH 6.7 and 30 degrees Celsius) {ECO:0000269|PubMed:18245280}; KM=352 uM for formyl-CoA (with oxalate as acceptor at pH 6.7 and 30 degrees Celsius) {ECO:0000269|PubMed:18245280}; KM=510 uM for oxalate (with formyl-CoA as donor at pH 6.7 and 30 degrees Celsius) {ECO:0000269|PubMed:18245280}; KM=80 mM for succinate (with formyl-CoA as donor at pH 6.7 and 30 degrees Celsius) {ECO:0000269|PubMed:18245280}; Note=Kcat is 130 sec(-1) for CoA-transferase activity with formyl-CoA as substrate (with oxalate as acceptor at pH 6.7 and 30 degrees Celsius). Kcat is 5.3 sec(-1) for CoA-transferase activity with formyl-CoA as substrate (with succinate as acceptor at pH 6.7 and 30 degrees Celsius).; |
| Pathway: | |
Metabolic intermediate degradation; oxalate degradation; CO(2) and formate from oxalate: step 1/2. {ECO:0000255|HAMAP-Rule:MF_00742}. |
| Subunit: | |
Homodimer. {ECO:0000255|HAMAP-Rule:MF_00742, ECO:0000269|PubMed:12844490, ECO:0000269|PubMed:14993676}. |
| Induction: | |
By the acid response regulator EvgA. {ECO:0000269|PubMed:12694615, ECO:0000269|PubMed:23335415}. |
| Disruption phenotype: | |
Cells lacking this gene show a reduced acid tolerance response (ATR) during the adaptation phase. However the deletion of YfdW has no effect on survival in oxalate-containing challenge medium. {ECO:0000269|PubMed:23335415}. |
| Similarity: | |
Belongs to the CoA-transferase III family. Frc subfamily. {ECO:0000255|HAMAP-Rule:MF_00742, ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.