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UniProt functional annotation for P0AEK2 | ||
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| UniProt code: P0AEK2. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis. {ECO:0000269|PubMed:10629181, ECO:0000269|PubMed:14996818, ECO:0000269|PubMed:7592873, ECO:0000269|PubMed:8631920, ECO:0000269|PubMed:8910376}. |
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| Catalytic activity: | |
Reaction=a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + H(+) + NADPH; Xref=Rhea:RHEA:17397, Rhea:RHEA-COMP:9916, Rhea:RHEA- COMP:9945, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78776, ChEBI:CHEBI:78827; EC=1.1.1.100; Evidence={ECO:0000269|PubMed:10629181, ECO:0000269|PubMed:7592873, ECO:0000269|PubMed:8631920, ECO:0000269|PubMed:8910376}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:17399; Evidence={ECO:0000269|PubMed:10629181, ECO:0000269|PubMed:7592873, ECO:0000269|PubMed:8910376}; |
| Catalytic activity: | |
Reaction=3-oxobutanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxybutanoyl- [ACP] + NADP(+); Xref=Rhea:RHEA:41804, Rhea:RHEA-COMP:9625, Rhea:RHEA-COMP:9626, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78450, ChEBI:CHEBI:78451; Evidence={ECO:0000269|PubMed:10629181, ECO:0000269|PubMed:7592873, ECO:0000269|PubMed:8910376}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41805; Evidence={ECO:0000269|PubMed:10629181, ECO:0000269|PubMed:7592873, ECO:0000269|PubMed:8910376}; |
| Catalytic activity: | |
Reaction=3-oxopentanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxypentanoyl- [ACP] + NADP(+); Xref=Rhea:RHEA:55100, Rhea:RHEA-COMP:9939, Rhea:RHEA-COMP:14091, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78818, ChEBI:CHEBI:78983; Evidence={ECO:0000269|PubMed:10629181}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55101; Evidence={ECO:0000269|PubMed:10629181}; |
| Catalytic activity: | |
Reaction=3-oxohexanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxyhexanoyl- [ACP] + NADP(+); Xref=Rhea:RHEA:41824, Rhea:RHEA-COMP:9629, Rhea:RHEA-COMP:9630, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78456, ChEBI:CHEBI:78457; Evidence={ECO:0000269|PubMed:10629181}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41825; Evidence={ECO:0000269|PubMed:10629181}; |
| Catalytic activity: | |
Reaction=3-oxoheptanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxyheptanoyl- [ACP] + NADP(+); Xref=Rhea:RHEA:55104, Rhea:RHEA-COMP:9943, Rhea:RHEA-COMP:14092, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78824, ChEBI:CHEBI:78987; Evidence={ECO:0000269|PubMed:10629181}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55105; Evidence={ECO:0000269|PubMed:10629181}; |
| Catalytic activity: | |
Reaction=3-oxooctanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxyoctanoyl- [ACP] + NADP(+); Xref=Rhea:RHEA:41840, Rhea:RHEA-COMP:9633, Rhea:RHEA-COMP:9634, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78460, ChEBI:CHEBI:78461; Evidence={ECO:0000269|PubMed:10629181}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41841; Evidence={ECO:0000269|PubMed:10629181}; |
| Catalytic activity: | |
Reaction=3-oxononanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxynonanoyl- [ACP] + NADP(+); Xref=Rhea:RHEA:55108, Rhea:RHEA-COMP:9944, Rhea:RHEA-COMP:14093, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78826, ChEBI:CHEBI:78988; Evidence={ECO:0000269|PubMed:10629181}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55109; Evidence={ECO:0000269|PubMed:10629181}; |
| Catalytic activity: | |
Reaction=3-oxodecanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxydecanoyl- [ACP] + NADP(+); Xref=Rhea:RHEA:41856, Rhea:RHEA-COMP:9637, Rhea:RHEA-COMP:9638, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78464, ChEBI:CHEBI:78466; Evidence={ECO:0000269|PubMed:10629181, ECO:0000269|PubMed:8910376}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41857; Evidence={ECO:0000269|PubMed:10629181, ECO:0000269|PubMed:8910376}; |
| Catalytic activity: | |
Reaction=3-oxohexadecanoyl-[ACP] + H(+) + NADPH = (3R)- hydroxyhexadecanoyl-[ACP] + NADP(+); Xref=Rhea:RHEA:41904, Rhea:RHEA- COMP:9649, Rhea:RHEA-COMP:9650, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78478, ChEBI:CHEBI:78480; Evidence={ECO:0000269|PubMed:8910376}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41905; Evidence={ECO:0000269|PubMed:8910376}; |
| Catalytic activity: | |
Reaction=3-oxo-(9Z)-hexadecenoyl-[ACP] + H(+) + NADPH = (3R)-hydroxy- (9Z)-hexadecenoyl-[ACP] + NADP(+); Xref=Rhea:RHEA:54928, Rhea:RHEA- COMP:14038, Rhea:RHEA-COMP:14040, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:138406, ChEBI:CHEBI:138407; Evidence={ECO:0000269|PubMed:8910376}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:54929; Evidence={ECO:0000269|PubMed:8910376}; |
| Catalytic activity: | |
Reaction=4-methyl-3-oxopentanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxy-4- methylpentanoyl-[ACP] + NADP(+); Xref=Rhea:RHEA:55112, Rhea:RHEA- COMP:9940, Rhea:RHEA-COMP:14094, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78820, ChEBI:CHEBI:78984; Evidence={ECO:0000269|PubMed:10629181}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55113; Evidence={ECO:0000269|PubMed:10629181}; |
| Catalytic activity: | |
Reaction=5-methyl-3-oxohexanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxy-5- methylhexanoyl-[ACP] + NADP(+); Xref=Rhea:RHEA:55116, Rhea:RHEA- COMP:9941, Rhea:RHEA-COMP:14095, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78822, ChEBI:CHEBI:78986; Evidence={ECO:0000269|PubMed:10629181}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55117; Evidence={ECO:0000269|PubMed:10629181}; |
| Catalytic activity: | |
Reaction=4-methyl-3-oxohexanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxy-4- methylhexanoyl-[ACP] + NADP(+); Xref=Rhea:RHEA:55120, Rhea:RHEA- COMP:9942, Rhea:RHEA-COMP:14096, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78823, ChEBI:CHEBI:78985; Evidence={ECO:0000269|PubMed:10629181}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55121; Evidence={ECO:0000269|PubMed:10629181}; |
| Activity regulation: | |
Inhibited by cinnamic acid derivatives. {ECO:0000269|PubMed:18977209}. |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is between 6.0 and 7.0. {ECO:0000269|PubMed:4381013}; |
| Pathway: | |
Lipid metabolism; fatty acid biosynthesis. {ECO:0000269|PubMed:14996818, ECO:0000269|PubMed:7592873}. |
| Subunit: | |
Homotetramer. {ECO:0000269|PubMed:11669613, ECO:0000269|PubMed:15016358}. |
| Miscellaneous: | |
Calcium ions stabilize the structure, and may inhibit FabG activity by obstructing access to the active site. |
| Similarity: | |
Belongs to the short-chain dehydrogenases/reductases (SDR) family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.