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UniProt functional annotation for P45568 | ||
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| UniProt code: P45568. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4- phosphate (MEP). |
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| Catalytic activity: | |
Reaction=2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D- xylulose 5-phosphate + H(+) + NADPH; Xref=Rhea:RHEA:13717, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:57792, ChEBI:CHEBI:58262, ChEBI:CHEBI:58349; EC=1.1.1.267; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Note=Divalent cation. Prefers Mg(2+), Mn(2+) or Co(2+).; |
| Activity regulation: | |
Inhibited by fosmidomycin. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=250 uM for DXP {ECO:0000269|PubMed:10787409}; Note=Measured in the presence of manganese ions.; pH dependence: Optimum pH is 7.0-8.5. {ECO:0000269|PubMed:10787409}; Temperature dependence: Optimum temperature is 40-60 degrees Celsius. {ECO:0000269|PubMed:10787409}; |
| Pathway: | |
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 1/6. |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:11741911, ECO:0000269|PubMed:11872159, ECO:0000269|PubMed:12621040, ECO:0000269|PubMed:15339150, ECO:0000269|PubMed:15567415}. |
| Similarity: | |
Belongs to the DXR family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.