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PDBsum entry 1pyt
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Ternary complex (zymogen)
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PDB id
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1pyt
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Contents |
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94 a.a.
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309 a.a.
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248 a.a.
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251 a.a.
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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The three-Dimensional structure of the native ternary complex of bovine pancreatic procarboxypeptidase a with proproteinase e and chymotrypsinogen c.
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Authors
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F.X.Gomis-Rüth,
M.Gómez,
W.Bode,
R.Huber,
F.X.Avilés.
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Ref.
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Embo J, 1995,
14,
4387-4394.
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PubMed id
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Abstract
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The metalloexozymogen procarboxypeptidase A is mainly secreted in ruminants as a
ternary complex with zymogens of two serine endoproteinases, chymotrypsinogen C
and proproteinase E. The bovine complex has been crystallized, and its molecular
structure analysed and refined at 2.6 A resolution to an R factor of 0.198. In
this heterotrimer, the activation segment of procarboxypeptidase A essentially
clamps the other two subunits, which shield the activation sites of the former
from tryptic attack. In contrast, the propeptides of both serine proproteinases
are freely accessible to trypsin. This arrangement explains the sequential and
delayed activation of the constituent zymogens. Procarboxypeptidase A is
virtually identical to the homologous monomeric porcine form. Chymotrypsinogen C
displays structural features characteristic for chymotrypsins as well as
elastases, except for its activation domain; similar to bovine chymotrypsinogen
A, its binding site is not properly formed, while its surface located activation
segment is disordered. The proproteinase E structure is fully ordered and
strikingly similar to active porcine elastase; its specificity pocket is
occluded, while the activation segment is fixed to the molecular surface. This
first structure of a native zymogen from the proteinase E/elastase family does
not fundamentally differ from the serine proproteinases known so far.
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Added reference #1*
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Title
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Determination of hemihedral twinning and initial structural analysis of crystals of the procarboxypeptidase A ternary complex.
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Authors
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F.X.Gomis-Rüth,
I.Fita,
R.Kiefersauer,
R.Huber,
F.X.Avilés,
J.Navaza.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 1995,
51,
819-823.
[DOI no: ]
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PubMed id
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Figure 1.
Fig. 1. (a) Graphical representation for different twinning fractions a of
he theretical cumulative distribution funtion (Z, ot), where Z
epresents the intensity relative to the mean intesity, giving the
fraction of eflections having an intensity less than Z. This function,
for noncentrosymmetric reflections of crystals, is given by (3 and
4) in the text. Note that
N(Z, ct) = N[Z,
(I a]. (b) Experimental
N(Z)
curves. A comparison with the theoretical distribution for
ot = 0.0 (thickest, solid line) as a reference is included. Six
untwinned data sets arbitrarily chosen (R. Huber, unpublihed results;
hin lines) display an exponential habitus and are always located
bove the theoretical distribution for an untwinned crystal. TC data
(thick, soli line), so as a further data set (R. Huber, unpublished
results; thick dashed line) for which twinning was suspected, render a
sigmoid habitus and lie below the theoretical values.
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The above figure is
reproduced from the cited reference
with permission from the IUCr
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*Note, "added" references are those not in the PDB file but
which have either been obtained from the journal or suggested by the
author(s).
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Secondary reference #1
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Title
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Procarboxypeptidase a-S6 : detailed structure analysis of the constituting subunits and implications for their activation
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Authors
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F.X.Gomis-Ruth,
M.Gomez,
J.Vendrell,
S.Ventura,
W.Bode,
R.Huber,
F.X.Aviles.
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Ref.
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TO BE PUBLISHED ...
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