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PDBsum entry 1prc
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Photosynthetic reaction center
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PDB id
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1prc
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Contents |
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333 a.a.
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273 a.a.
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323 a.a.
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258 a.a.
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Crystallographic refinement at 2.3 a resolution and refined model of the photosynthetic reaction centre from rhodopseudomonas viridis.
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Authors
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J.Deisenhofer,
O.Epp,
I.Sinning,
H.Michel.
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Ref.
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J Mol Biol, 1995,
246,
429-457.
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PubMed id
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Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
percentage match of
95%.
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Abstract
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The atomic model of the photosynthetic reaction centre from the purple bacterium
Rhodopseudomonas viridis has been refined to an R-value of 0.193 at 2.3 A
resolution. The refined model contains 10,288 non-hydrogen atoms; 10,045 of
these have well defined electron density. A Luzzati-plot indicates an average
co-ordinate error of 0.26 A. During refinement, the positions of a partially
ordered carotenoid, a unibiquinone in the partially occupied QB site, a
detergent molecule, seven putative sulphate ions, and 201 water molecules were
found. More than half of these waters are bound at interfaces between protein
subunits and therefore contribute significantly to subunit interactions. Water
molecules also play important structural and probably functional roles in the
environment of some of the cofactors. Two water molecules form hydrogen bonds to
the accessory bacteriochlorophylls and to the protein in the vicinity of the
special pair of bacteriophylls, the primary electron donor. A group of about 10
water molecules is bound near the binding site of the secondary quinone QB.
These waters are likely to participate in the transfer of protons to the doubly
reduced QB.
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Secondary reference #1
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Title
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The photosynthetic reaction center from the purple bacterium rhodopseudomonas viridis.
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Authors
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J.Deisenhofer,
H.Michel.
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Ref.
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Science, 1989,
245,
1463-1473.
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PubMed id
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Secondary reference #2
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Title
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Structure of the protein subunits in the photosynthetic reaction centre of rhodopseudomonas viridis at 3 angstroms resolution
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Authors
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J.Deisenhofer,
O.Epp,
K.Miki,
R.Huber,
H.Michel.
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Ref.
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nature, 1985,
318,
618.
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Secondary reference #3
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Title
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X-Ray structure analysis of a membrane protein complex. Electron density map at 3 a resolution and a model of the chromophores of the photosynthetic reaction center from rhodopseudomonas viridis.
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Authors
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J.Deisenhofer,
O.Epp,
K.Miki,
R.Huber,
H.Michel.
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Ref.
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J Mol Biol, 1984,
180,
385-398.
[DOI no: ]
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PubMed id
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Figure 3.
Fro. 3. Stereo draing of the special pair. The central local symmetry ais runs
between the BChl-bs. Ring numbers are indicated in one BChl-b. Phytyl chains are truncated.
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Figure 4.
FIG. 4. Stereo drawing of the prosthetic grups of the RC shwing 4 BChl-b (BC), 2 BPh-b (BP), 1
non-eine iron (Fe), 1 quinone (MQ) an heine grups (HE). The central local symmetry axis runs
vertically in the plane of the picture. The plane of the membane is assumed t be oriented
perpendicular to the central local symmetry axis, i.e. horizontal and perpendicular to the plane of th
picture. The dotted lines marked 0 and I indicate the presmed approximate outer and inner
membrane surfaces of the bacterial cell. The BChl-b, BPh-b, quinone and iron are lcated within th
cylindrical central part of the RC compex; te ~ 70 A diameter of the cylinder is perpendicular to th
local symmetry axis in the plane of the picture
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The above figures are
reproduced from the cited reference
with permission from Elsevier
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Secondary reference #4
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Title
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Three-Dimensional crystals of a membrane protein complex. The photosynthetic reaction centre from rhodopseudomonas viridis.
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Author
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H.Michel.
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Ref.
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J Mol Biol, 1982,
158,
567-572.
[DOI no: ]
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PubMed id
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Figure 1.
IG:. 1. Elation profile of the molecular siev chromatograph step used for the isolation of thr
photosvnthetic reaction centrrs from tl. Gridis. I'hotosynthrtir membranes (I6 mg of protein/ml) were
solubil&d in 5''' f. w/v) S.K~dod~c?ildimet,h~lamine A-oxide. WI O,> (y/v) Sx3. 10 mwTris_ HCI (pH 7)
at room temprratre for 5 mill. After a clarifying spin (1OO,OOOg, 30 min). 09.5 ml of the supwnatant
was applird to a. TSK 3ooO SW column (LKH. (ZrLfelfing) and run nt 0.1 ml/in in 20 mM-sodium
phosphat,r (pH 7). 0.1 `)o (w/r) S,S-dodervldimc,th?aminr A-oxide, PI'',, (W/V) NaN,. The reaction
crntres form the prak arktad I%(`.
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The above figure is
reproduced from the cited reference
with permission from Elsevier
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