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UniProt functional annotation for P96202 | ||
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| UniProt code: P96202. |
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| Organism: | |
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv). |
| Taxonomy: | |
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. |
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| Function: | |
Part of the PpsABCDE complex involved in the biosynthesis of the lipid core common to phthiocerols and phenolphthiocerols by successive additions of malonyl-CoA or methylmalonyl-CoA extender units (PubMed:15749014, PubMed:20553505). PpsA can accept as substrate the activated forms of either icosanoyl (C20), docosanoyl (C22) or lignoceroyl (C24) groups from FadD26, or a (4-hydroxyphenyl)-C17 or (4- hydroxyphenyl)-C19 fatty acyl from FadD29 (PubMed:15749014, PubMed:20553505). PpsA initiates the biosynthesis and extends its substrate using a malonyl-CoA extender unit. The PpsB and PpsC proteins add the second and third malonyl-CoA extender units. PpsD adds an (R)- methylmalonyl unit and PpsE adds a second (R)-methylmalonyl unit. The incorporation of the methylmalonyl units results in formation of two branched methyl groups in the elongated product (PubMed:15749014). {ECO:0000269|PubMed:15749014, ECO:0000269|PubMed:20553505}. |
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| Catalytic activity: | |
Reaction=2 (S)-methylmalonyl-CoA + 10 H(+) + icosanoyl- [(phenol)carboxyphthiodiolenone synthase] + 3 malonyl-CoA + 5 NADPH = C32-carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CO2 + 5 CoA + 2 H2O + 5 NADP(+); Xref=Rhea:RHEA:57748, Rhea:RHEA- COMP:14985, Rhea:RHEA-COMP:14986, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57327, ChEBI:CHEBI:57384, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:87848, ChEBI:CHEBI:142236; EC=2.3.1.292; Evidence={ECO:0000269|PubMed:15749014, ECO:0000305|PubMed:20553505}; |
| Catalytic activity: | |
Reaction=2 (S)-methylmalonyl-CoA + docosanoyl- [(phenol)carboxyphthiodiolenone synthase] + 10 H(+) + 3 malonyl-CoA + 5 NADPH = C34-carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CO2 + 5 CoA + 2 H2O + 5 NADP(+); Xref=Rhea:RHEA:57752, Rhea:RHEA-COMP:14987, Rhea:RHEA-COMP:14988, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57327, ChEBI:CHEBI:57384, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:142237, ChEBI:CHEBI:142238; EC=2.3.1.292; Evidence={ECO:0000269|PubMed:15749014, ECO:0000305|PubMed:20553505}; |
| Catalytic activity: | |
Reaction=2 (S)-methylmalonyl-CoA + 17-(4-hydroxyphenyl)heptadecanoyl- [(phenol)carboxyphthiodiolenone synthase] + 10 H(+) + 3 malonyl-CoA + 5 NADPH = C35-(phenol)carboxyphthiodiolenone- [(phenol)carboxyphthiodiolenone synthase] + 5 CO2 + 5 CoA + 2 H2O + 5 NADP(+); Xref=Rhea:RHEA:57756, Rhea:RHEA-COMP:14272, Rhea:RHEA- COMP:14989, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57327, ChEBI:CHEBI:57384, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133300, ChEBI:CHEBI:142259; EC=2.3.1.292; Evidence={ECO:0000269|PubMed:15749014, ECO:0000305|PubMed:20553505}; |
| Catalytic activity: | |
Reaction=2 (S)-methylmalonyl-CoA + 19-(4-hydroxyphenyl)nonadecanoyl- [(phenol)carboxyphthiodiolenone synthase] + 10 H(+) + 3 malonyl-CoA + 5 NADPH = C37-(phenol)carboxyphthiodiolenone- [(phenol)carboxyphthiodiolenone synthase] + 5 CO2 + 5 CoA + 2 H2O + 5 NADP(+); Xref=Rhea:RHEA:57760, Rhea:RHEA-COMP:14273, Rhea:RHEA- COMP:14990, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57327, ChEBI:CHEBI:57384, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133301, ChEBI:CHEBI:142260; EC=2.3.1.292; Evidence={ECO:0000269|PubMed:15749014, ECO:0000305|PubMed:20553505}; |
| Cofactor: | |
Name=NADP(+); Xref=ChEBI:CHEBI:58349; Evidence={ECO:0000269|PubMed:15749014}; |
| Cofactor: | |
Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942; Evidence={ECO:0000269|PubMed:28203522}; Note=Binds 1 phosphopantetheine covalently. {ECO:0000305|PubMed:28203522}; |
| Pathway: | |
Lipid metabolism; fatty acid biosynthesis. {ECO:0000269|PubMed:15749014}. |
| Subunit: | |
Homodimer. {ECO:0000269|Ref.7}. |
| Miscellaneous: | |
Was identified as a high-confidence drug target. {ECO:0000269|PubMed:19099550}. |
Annotations taken from UniProtKB at the EBI.