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UniProt functional annotation for P08308 | ||
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| UniProt code: P08308. |
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| Organism: | |
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. |
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| Function: | |
Involved in the catabolism of arginine. Catalyzes the phosphorolysis of citrulline, the reverse reaction of the biosynthetic one, yielding ornithine and carbamoyl phosphate which serve to generate ATP from ADP (PubMed:4962140, PubMed:2118516). This catabolic OTCase does not carry out the biosynthetic reaction because of a poor affinity and a marked cooperativity for carbamoyl phosphate (PubMed:2118516). {ECO:0000269|PubMed:2118516, ECO:0000269|PubMed:4962140, ECO:0000305|PubMed:113384, ECO:0000305|PubMed:3109911, ECO:0000305|PubMed:8102605}. |
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| Catalytic activity: | |
Reaction=carbamoyl phosphate + L-ornithine = H(+) + L-citrulline + phosphate; Xref=Rhea:RHEA:19513, ChEBI:CHEBI:15378, ChEBI:CHEBI:43474, ChEBI:CHEBI:46911, ChEBI:CHEBI:57743, ChEBI:CHEBI:58228; EC=2.1.3.3; Evidence={ECO:0000255|HAMAP- Rule:MF_01109}; |
| Activity regulation: | |
Inhibited by 2-aminopentanoic acid (norvaline). Activated by phosphate and nucleoside monophosphates such as AMP, GMP, CMP, UMP. Allosterically inhibited by the polyamines such as spermidine and putrescine. {ECO:0000269|PubMed:8102605}. |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is 7.3. {ECO:0000269|PubMed:4962140}; |
| Pathway: | |
Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 2/2. {ECO:0000305|PubMed:113384, ECO:0000305|PubMed:4962140, ECO:0000305|PubMed:6438064}. |
| Subunit: | |
Nonameric or dodecamer (tetramer of trimers). {ECO:0000269|PubMed:10489456, ECO:0000269|PubMed:3109911, ECO:0000269|PubMed:7479879, ECO:0000269|Ref.11}. |
| Subcellular location: | |
Cytoplasm {ECO:0000305}. |
| Induction: | |
During limiting aeration and in the presence of arginine. {ECO:0000269|PubMed:6252188, ECO:0000305|PubMed:3109911}. |
| Disruption phenotype: | |
Cells lacking this gene are blocked in the arginine deiminase pathway. {ECO:0000269|PubMed:113384}. |
| Miscellaneous: | |
Proceeds by an ordered sequential mechanism with CP identified as the initial reactant. {ECO:0000305|PubMed:8102605}. |
| Similarity: | |
Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.