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UniProt functional annotation for Q00955 | ||
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| UniProt code: Q00955. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. Involved in the synthesis of very-long-chain fatty acid synthesis which is required to maintain a functional nuclear envelope. Required for acylation and vacuolar membrane association of VAC8 which is necessary to maintain a normal morphology of the vacuole. {ECO:0000269|PubMed:10757783, ECO:0000269|PubMed:12730220, ECO:0000269|PubMed:6103540, ECO:0000269|PubMed:6108218, ECO:0000269|PubMed:8943372}. |
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| Catalytic activity: | |
Reaction=acetyl-CoA + ATP + hydrogencarbonate = ADP + H(+) + malonyl- CoA + phosphate; Xref=Rhea:RHEA:11308, ChEBI:CHEBI:15378, ChEBI:CHEBI:17544, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57288, ChEBI:CHEBI:57384, ChEBI:CHEBI:456216; EC=6.4.1.2; Evidence={ECO:0000269|PubMed:12663926, ECO:0000269|PubMed:15079078}; |
| Catalytic activity: | |
Reaction=ATP + hydrogencarbonate + N(6)-biotinyl-L-lysyl-[protein] = ADP + H(+) + N(6)-carboxybiotinyl-L-lysyl-[protein] + phosphate; Xref=Rhea:RHEA:13501, Rhea:RHEA-COMP:10505, Rhea:RHEA-COMP:10506, ChEBI:CHEBI:15378, ChEBI:CHEBI:17544, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:83144, ChEBI:CHEBI:83145, ChEBI:CHEBI:456216; EC=6.3.4.14; Evidence={ECO:0000250|UniProtKB:Q5SWU9}; |
| Cofactor: | |
Name=biotin; Xref=ChEBI:CHEBI:57586; Evidence={ECO:0000250|UniProtKB:O00763}; |
| Cofactor: | |
Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250}; Note=Binds 2 manganese ions per subunit. {ECO:0000250}; |
| Activity regulation: | |
By phosphorylation. The catalytic activity is inhibited by soraphen A, a polyketide isolated from the myxobacterium Sorangium cellulosum and a potent inhibitor of fungal growth. {ECO:0000269|PubMed:7916271}. |
| Pathway: | |
Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1. |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:12663926, ECO:0000269|PubMed:15079078, ECO:0000269|PubMed:15610732}. |
| Subcellular location: | |
Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. |
| Induction: | |
Repressed in presence of fatty acids. Repressed 3-fold by lipid precursors, inositol and choline, and also controlled by regulatory factors INO2, INO4 and OPI1. {ECO:0000269|PubMed:1359536, ECO:0000269|PubMed:8098706, ECO:0000269|PubMed:8127678}. |
| Miscellaneous: | |
Present with 20200 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
Annotations taken from UniProtKB at the EBI.