UniProt functional annotation for P06278

UniProt code: P06278.

Organism: Bacillus licheniformis.
Taxonomy: Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
 
Catalytic activity: Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D- glucose units.; EC=3.2.1.1; Evidence={ECO:0000269|PubMed:11997021, ECO:0000269|PubMed:12915728, ECO:0000269|PubMed:14632998};
Cofactor: Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000269|PubMed:12540849, ECO:0000269|PubMed:9551551}; Note=Binds 3 Ca(2+) ions per subunit. {ECO:0000269|PubMed:12540849, ECO:0000269|PubMed:9551551};
Cofactor: Name=Na(+); Xref=ChEBI:CHEBI:29101; Evidence={ECO:0000269|PubMed:12540849, ECO:0000269|PubMed:9551551}; Note=Binds 1 sodium ion per subunit. {ECO:0000269|PubMed:12540849, ECO:0000269|PubMed:9551551};
Biophysicochemical properties: pH dependence: Active up to pH 11.; Temperature dependence: Active up to 100 degrees Celsius.;
Subunit: Monomer.
Subcellular location: Secreted {ECO:0000269|PubMed:14632998}.
Biotechnology: Used in the food industry for high temperature liquefaction of starch-containing mashes and in the detergent industry to remove starch. Sold under the name Termamyl by Novozymes.
Similarity: Belongs to the glycosyl hydrolase 13 family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.