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UniProt functional annotation for P0A110 | ||
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| UniProt code: P0A110. |
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| Organism: | |
Pseudomonas putida (Arthrobacter siderocapsulatus). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. |
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| Function: | |
Component of the naphthalene dioxygenase (NDO) multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into naphthalene to form cis-(1R,2S)-dihydroxy-1,2- dihydronaphthalene. The alpha subunit has a catalytic role in the holoenzyme. Also able to catalyze the cis-dihydroxylation of biphenyl and phenanthrene (PubMed:10692370). {ECO:0000269|PubMed:10692370, ECO:0000269|PubMed:6874638}. |
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| Catalytic activity: | |
Reaction=H(+) + NADH + naphthalene + O2 = (1R,2S)-1,2- dihydronaphthalene-1,2-diol + NAD(+); Xref=Rhea:RHEA:19173, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16482, ChEBI:CHEBI:44343, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.14.12.12; Evidence={ECO:0000269|PubMed:10692370, ECO:0000269|PubMed:6874638}; |
| Cofactor: | |
Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:49601; Evidence={ECO:0000269|PubMed:10669618, ECO:0000269|PubMed:12586937, ECO:0000269|PubMed:15942729, ECO:0000269|PubMed:6874638, ECO:0000269|PubMed:9634695}; Note=Binds 1 [2Fe-2S] cluster. {ECO:0000269|PubMed:10669618, ECO:0000269|PubMed:12586937, ECO:0000269|PubMed:15942729, ECO:0000269|PubMed:6874638, ECO:0000269|PubMed:9634695}; |
| Cofactor: | |
Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000269|PubMed:10669618, ECO:0000269|PubMed:12586937, ECO:0000269|PubMed:15942729, ECO:0000269|PubMed:9634695}; Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000269|PubMed:10669618, ECO:0000269|PubMed:12586937, ECO:0000269|PubMed:15942729, ECO:0000269|PubMed:9634695}; |
| Pathway: | |
Aromatic compound metabolism; naphthalene degradation. {ECO:0000305|PubMed:10692370}. |
| Subunit: | |
The naphthalene dioxygenase (NDO) multicomponent enzyme system is composed of an electron transfer component and a dioxygenase component (iron sulfur protein (ISP)). The electron transfer component is composed of a ferredoxin reductase (NdoR) and a ferredoxin (NdoA), and the dioxygenase component is formed of a heterohexamer (trimer of heterodimers) of three large alpha subunits (NdoB) and three small beta subunits (NdoC). {ECO:0000269|PubMed:12586937, ECO:0000269|PubMed:15942729, ECO:0000269|PubMed:9634695, ECO:0000305|PubMed:6874638}. |
| Similarity: | |
Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.