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PDBsum entry 1nrt
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Amino-acid transport
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PDB id
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1nrt
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References listed in PDB file
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Key reference
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Title
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A model of protein synthesis based on cryo-Electron microscopy of the e. Coli ribosome.
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Authors
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J.Frank,
J.Zhu,
P.Penczek,
Y.Li,
S.Srivastava,
A.Verschoor,
M.Radermacher,
R.Grassucci,
R.K.Lata,
R.K.Agrawal.
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Ref.
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Nature, 1995,
376,
441-444.
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PubMed id
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Abstract
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The ribosome is formed by assembly of proteins and nucleic acids, and
synthesizes proteins according to genetic instructions in all organisms. Many of
the biochemical steps of this fundamental process are known, but a detailed
understanding requires a well-defined structural model of the ribosome. Electron
microscopy combined with image reconstruction of two-dimensional crystals or
single ribosomes has been the most promising technique, but the resolution of
the resulting models has been insufficient. Here we report a 25-A reconstruction
of the ribosome from Escherichia coli, obtained by combining 4,300 projections
of ice-embedded single particles. Our new reconstruction reveals a channel in
the small ribosomal subunit and a bifurcating tunnel in the large subunit which
may constitute pathways for the incoming message and the nascent polypeptide
chain, respectively. Based on these new findings, a three-dimensional model of
the basic framework of protein synthesis is presented.
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Secondary reference #1
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Title
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Direct visualization of a-, P-, And e-Site transfer rnas in the escherichia coli ribosome.
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Authors
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R.K.Agrawal,
P.Penczek,
R.A.Grassucci,
Y.Li,
A.Leith,
K.H.Nierhaus,
J.Frank.
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Ref.
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Science, 1996,
271,
1000-1002.
[DOI no: ]
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PubMed id
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