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PDBsum entry 1nap
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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The crystal structure of recombinant human neutrophil-Activating peptide-2 (m6l) at 1.9-A resolution.
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Authors
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M.G.Malkowski,
J.Y.Wu,
J.B.Lazar,
P.H.Johnson,
B.F.Edwards.
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Ref.
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J Biol Chem, 1995,
270,
7077-7087.
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PubMed id
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Abstract
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Neutrophil-activating peptide-2 (NAP-2) is a 70-residue carboxyl-terminal
fragment of platelet basic protein, which is found in the alpha-granules of
human platelets. NAP-2, which belongs to the CXC family of chemokines that
includes interleukin-8 and platelet factor 4, binds to the interleukin-8 type II
receptor and induces a rise in cytosolic calcium, chemotaxis of neutrophils, and
exocytosis. Crystals of recombinant NAP-2 in which the single methionine at
position 6 was replaced by leucine to facilitate expression belong to space
group P1 (unit cell parameters a = 40.8, b = 43.8, and c = 44.7 A and alpha =
98.4 degrees, beta = 120.3 degrees, and gamma = 92.8 degrees), with 4 molecules
of NAP-2 (Mr = 7600) in the asymmetric unit. The molecular replacement solution
calculated with bovine platelet factor 4 as the starting model was refined using
rigid body refinement, manual fitting in solvent-leveled electron density maps,
simulated annealing, and restrained least squares to an R-factor of 0.188 for 2
sigma data between 7.0- and 1.9-A resolution. The final refined crystal
structure includes 265 solvent molecules. The overall tertiary structure, which
is similar to that of platelet factor 4 and interleukin-8, includes an extended
amino-terminal loop, three strands of antiparallel beta-sheet arranged in a
Greek key fold, and one alpha-helix at the carboxyl terminus. The Glu-Leu-Arg
sequence that is critical for receptor binding is fully defined by electron
density and exhibits multiple conformations.
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Secondary reference #1
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Title
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The three-Dimensional structure of bovine platelet factor 4 at 3.0-A resolution.
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Authors
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R.St charles,
D.A.Walz,
B.F.Edwards.
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Ref.
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J Biol Chem, 1989,
264,
2092-2099.
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PubMed id
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Secondary reference #2
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Title
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Crystal structure of interleukin 8: symbiosis of nmr and crystallography.
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Authors
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E.T.Baldwin,
I.T.Weber,
R.St charles,
J.C.Xuan,
E.Appella,
M.Yamada,
K.Matsushima,
B.F.Edwards,
G.M.Clore,
A.M.Gronenborn.
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Ref.
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Proc Natl Acad Sci U S A, 1991,
88,
502-506.
[DOI no: ]
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PubMed id
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Secondary reference #3
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Title
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Three-Dimensional structure of interleukin 8 in solution.
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Authors
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G.M.Clore,
E.Appella,
M.Yamada,
K.Matsushima,
A.M.Gronenborn.
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Ref.
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Biochemistry, 1990,
29,
1689-1696.
[DOI no: ]
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PubMed id
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