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UniProt functional annotation for Q46857 | ||
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| UniProt code: Q46857. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). It is also capable of stereoselective -keto ester reductions on ethyl acetoacetate and other 2-substituted derivatives. |
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| Catalytic activity: | |
Reaction=2-dehydro-D-gluconate + NADP(+) = 2,5-didehydro-D-gluconate + H(+) + NADPH; Xref=Rhea:RHEA:23828, ChEBI:CHEBI:11449, ChEBI:CHEBI:15378, ChEBI:CHEBI:16808, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.274; |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is 7.5.; |
| Subunit: | |
Monomer. |
| Subcellular location: | |
Cytoplasm {ECO:0000250}. |
| Miscellaneous: | |
2-keto-L-gulonic acid is a key intermediate in the production of L-ascorbic acid (vitamin C). |
| Similarity: | |
Belongs to the aldo/keto reductase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.