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PDBsum entry 1mzb
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Gene regulation
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PDB id
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1mzb
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References listed in PDB file
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Key reference
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Title
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Architecture of a protein central to iron homeostasis: crystal structure and spectroscopic analysis of the ferric uptake regulator.
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Authors
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E.Pohl,
J.C.Haller,
A.Mijovilovich,
W.Meyer-Klaucke,
E.Garman,
M.L.Vasil.
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Ref.
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Mol Microbiol, 2003,
47,
903-915.
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PubMed id
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Abstract
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Iron is an essential element for almost all organisms, although an overload of
this element results in toxicity because of the formation of hydroxyl radicals.
Consequently, most living entities have developed sophisticated mechanisms to
control their intracellular iron concentration. In many bacteria, including the
opportunistic pathogen Pseudomonas aeruginosa, this task is performed by the
ferric uptake regulator (Fur). Fur controls a wide variety of basic
physiological processes including iron uptake systems and the expression of
exotoxin A. Here, we present the first crystal structure of Fur from P.
aeruginosa in complex with Zn2+ determined at a resolution of 1.8 A.
Furthermore, X-ray absorption spectroscopic measurements and microPIXE analysis
were performed in order to characterize the distinct zinc and iron binding sites
in solution. The combination of these complementary techniques enables us to
present a model for the activation and DNA binding of the Fur protein.
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