PDBsum entry 1mtk

Oxygen storage

PDB id: 1mtk

Contents

Protein chain

154 a.a.

Ligands

SO4

HEM

Waters ×133

References listed in PDB file

Key reference

• Title: Phe-46(cd4) orients the distal histidine for hydrogen bonding to bound ligands in sperm whale myoglobin.
• Authors: H.H.Lai, T.Li, D.S.Lyons, G.N.Phillips, J.S.Olson, Q.H.Gibson.
• Ref.: Proteins, 1995, 22, 322-339.
• PubMed id: 7479707

Note In the PDB file this reference is annotated as "TO BE PUBLISHED". The citation details given above were identified by an automated search of PubMed on title and author names, giving a percentage match of 91%.

Abstract

The role of Phe-46(CD4) in modulating the functional properties of sperm whale myoglobin was investigated by replacing this residue with Leu, Ile, Val, Ala, Trp, Tyr, and Glu. This highly conserved amino acid almost makes direct contact with the distal histidine and has been postulated to affect ligand binding. The overall association rate constants for CO, O2, and NO binding were little affected by decreasing the size of residue 46 step-wise from Phe to Leu to Val to Ala. In contrast, the rates of CO, O2, and NO dissociation increased 4-, 10-, and 25-fold, respectively, for the same series of mutants, causing large decreases in the affinity of myoglobin for all three diatomic gases. The rates of autooxidation at 37 degrees C, pH 7.0 increased dramatically from approximately 0.1-0.3 h-1 for wild-type, Tyr-46, and Trp-46 myoglobins to 1.5, 5.2, 4.9, and 5.0 h-1 for the Leu-46, Ile-46, Val-46 and Ala-46 mutants, respectively. Rates of NO and O2 geminate recombination were measured using 35 ps and 9 ns laser excitation pulses. Decreasing the size of residue 46 causes significant decreases in the extent of both picosecond and nanosecond rebinding processes. High resolution structures of Leu-46 and Val-46 metmyoglobins, Val-46 CO-myoglobin, and Val-46 deoxymyoglobin were determined by X-ray crystallography. When Phe-46 is replaced by Val, the loss of internal packing volume is compensated by (1) contraction of the CD corner toward the core of the protein, (2) movement of the E-helix toward the mutation site, (3) greater exposure of the distal pocket to intruding solvent molecules, and (4) large disorder in the position of the side chain of the distal histidine (His-64). In wild-type myoglobin, the van der Waals contact between C zeta of Phe-46 and C beta of His-64 appears to restrict rotation of the imidazole side chain. Insertion of Val at position 46 relieves this steric restriction, allowing the imidazole side chain to rotate about the C alpha - C beta bond toward the surface of the globin and about the C beta - C gamma bond toward the space previously occupied by the native Phe-46 side chain. This movement disrupts hydrogen bonding with bound ligands, causing significant decreases in affinity, and opens the distal pocket to solvent water molecules, causing marked increases in the rate of autooxidation.(ABSTRACT TRUNCATED AT 400 WORDS)

Secondary reference #1

• Title: High-Resolution crystal structures of distal histidine mutants of sperm whale myoglobin.
• Authors: M.L.Quillin, R.M.Arduini, J.S.Olson, G.N.Phillips.
• Ref.: J Mol Biol, 1993, 234, 140-155.
• PubMed id: 8230194

Secondary reference #2

• Title: Crystal structure of myoglobin from a synthetic gene.
• Authors: G.N.Phillips, R.M.Arduini, B.A.Springer, S.G.Sligar.
• Ref.: Proteins, 1990, 7, 358-365.
• PubMed id: 2199973