UniProt functional annotation for P00787



	UniProt functional annotation for P00787

UniProt code: P00787.

Organism: Rattus norvegicus (Rat).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus.

Function: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (PubMed:1639824). Cleaves matrix extracellular phosphoglycoprotein MEPE (By similarity). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity). Has also been implicated in tumor invasion and metastasis (By similarity). {ECO:0000250|UniProtKB:P07858, ECO:0000250|UniProtKB:P10605, ECO:0000269|PubMed:1639824}.

Catalytic activity: Reaction=Hydrolysis of proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C- terminal dipeptides.; EC=3.4.22.1; Evidence={ECO:0000269|PubMed:1639824};

Subunit: Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. Directly interacts with SHKBP1. {ECO:0000250|UniProtKB:P07858}.

Subcellular location: Lysosome {ECO:0000269|PubMed:8001549}. Melanosome {ECO:0000250|UniProtKB:P07858}. Secreted, extracellular space {ECO:0000250|UniProtKB:A1E295}. Apical cell membrane {ECO:0000250|UniProtKB:P10605}; Peripheral membrane protein {ECO:0000250|UniProtKB:P10605}; Extracellular side {ECO:0000250|UniProtKB:P10605}. Note=Localizes to the lumen of thyroid follicles and to the apical membrane of thyroid epithelial cells. {ECO:0000250|UniProtKB:P10605}.

Tissue specificity: Expressed in the epithelial cells of the prostate and mammary gland. {ECO:0000269|PubMed:8001549}.

Induction: Expression is low in the lactacting mammary gland but increases after weaning to a peak 4 days post weaning. Expression returns to baseline levels 6 days post weaning. {ECO:0000269|PubMed:8001549}.

Similarity: Belongs to the peptidase C1 family. {ECO:0000255|PROSITE- ProRule:PRU10088, ECO:0000255|PROSITE-ProRule:PRU10089, ECO:0000255|PROSITE-ProRule:PRU10090}.

Annotations taken from UniProtKB at the EBI.


Organism:		Rattus norvegicus (Rat).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus.



Function:		Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (PubMed:1639824). Cleaves matrix extracellular phosphoglycoprotein MEPE (By similarity). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity). Has also been implicated in tumor invasion and metastasis (By similarity). {ECO:0000250\|UniProtKB:P07858, ECO:0000250\|UniProtKB:P10605, ECO:0000269\|PubMed:1639824}.


Catalytic activity:		Reaction=Hydrolysis of proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg-\|-Xaa bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C- terminal dipeptides.; EC=3.4.22.1; Evidence={ECO:0000269\|PubMed:1639824};
Subunit:		Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. Directly interacts with SHKBP1. {ECO:0000250\|UniProtKB:P07858}.
Subcellular location:		Lysosome {ECO:0000269\|PubMed:8001549}. Melanosome {ECO:0000250\|UniProtKB:P07858}. Secreted, extracellular space {ECO:0000250\|UniProtKB:A1E295}. Apical cell membrane {ECO:0000250\|UniProtKB:P10605}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P10605}; Extracellular side {ECO:0000250\|UniProtKB:P10605}. Note=Localizes to the lumen of thyroid follicles and to the apical membrane of thyroid epithelial cells. {ECO:0000250\|UniProtKB:P10605}.
Tissue specificity:		Expressed in the epithelial cells of the prostate and mammary gland. {ECO:0000269\|PubMed:8001549}.
Induction:		Expression is low in the lactacting mammary gland but increases after weaning to a peak 4 days post weaning. Expression returns to baseline levels 6 days post weaning. {ECO:0000269\|PubMed:8001549}.
Similarity:		Belongs to the peptidase C1 family. {ECO:0000255\|PROSITE- ProRule:PRU10088, ECO:0000255\|PROSITE-ProRule:PRU10089, ECO:0000255\|PROSITE-ProRule:PRU10090}.