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PDBsum entry 1lzt
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Hydrolase(o-glycosyl)
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PDB id
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1lzt
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References listed in PDB file
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Key reference
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Title
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Refinement of triclinic lysozyme: i. Fourier and least-Squares methods.
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Authors
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J.M.Hodsdon,
G.M.Brown,
L.C.Sieker,
L.H.Jensen.
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Ref.
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Acta Crystallogr B, 1990,
46,
54-62.
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PubMed id
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Abstract
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X-ray diffraction data to 1.5 A resolution have been collected for triclinic
crystals of hen egg white lysozyme. The triclinic model was derived from the
tetragonal one by the rotation function and refined initially by Fo-Fc and
differential difference syntheses against 2 A resolution data. Refinement was
continued by differential difference cycles against the 1.5 A data until R was
reduced to 0.220. Although the initial refinement was rapid, it was subsequently
a matter of attrition, leading to a complete recheck of the data and the
discovery of systematic error which affected primarily the high-resolution data.
Refinement was continued against the corrected 2 A data by block-diagonal least
squares. After five cycles the refinement was terminated at R = 0.254 because of
the imminent availability of a preferred refinement program. Problems with the
protein model, the solvent, and the interaction of the scale and thermal
parameters are discussed. The experiences gained in this study are summarized.
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Secondary reference #1
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Title
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Structures of triclinic mono- And di-N-Acetylglucosamine: lysozyme complexes--A crystallographic study.
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Authors
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K.Kurachi,
L.C.Sieker,
L.H.Jensen.
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Ref.
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J Mol Biol, 1976,
101,
11-24.
[DOI no: ]
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PubMed id
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Figure 1.
VIU. 1. Sterzo view of GalNAc : LYZ cliffiirence map at 2 A resolution. Contours at intervals
f 0.1 eam3; light contours, Iwgative; dark contours, positive: zero contour omitted. Phases from
t+inement of native lysozymr at R = 0.274. ? .i data.
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Figure 7.
FIG. 7. Distances between some prtein and di-GrtlNAc atoms in di-GalNAc : LYZ.
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The above figures are
reproduced from the cited reference
with permission from Elsevier
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