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PDBsum entry 1lyd
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Hydrolase (o-glycosyl)
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PDB id
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1lyd
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References listed in PDB file
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Key reference
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Title
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Crystal structure of t4-Lysozyme generated from synthetic coding DNA expressed in escherichia coli.
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Authors
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D.R.Rose,
J.Phipps,
J.Michniewicz,
G.I.Birnbaum,
F.R.Ahmed,
A.Muir,
W.F.Anderson,
S.Narang.
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Ref.
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Protein Eng, 1988,
2,
277-282.
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PubMed id
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Abstract
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The polypeptide produced by expressing a chemically synthesized gene coding for
the amino-acid sequence of T4-lysozyme has been crystallized and subjected to
X-ray diffraction. The crystal structure has been refined to a standard R-factor
of 0.191 for data between 8 and 2 A resolution. The refined model is essentially
the same as the well-known structure of wild-type T4-lysozyme determined
previously by Matthews et al. (1987). Some small changes in the C-terminal
region, which is important in maintaining the folded structure, have been noted.
In addition to confirming that the synthetic gene product is very close to the
wild type, this structure provides a benchmark for protein engineering
experiments on the folding and the catalytic activity of this molecule by the
method of gene synthesis.
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Secondary reference #1
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Title
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Hierarchical strategy for protein folding and design: synthesis and expression of t4 lysozyme gene and two putative folding mutants.
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Authors
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S.A.Narang,
F.L.Yao,
J.J.Michniewicz,
G.Dubuc,
J.Phipps,
R.L.Somorjai.
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Ref.
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Protein Eng, 1987,
1,
481-485.
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PubMed id
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Secondary reference #2
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Title
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Structure of bacteriophage t4 lysozyme refined at 1.7 angstroms resolution
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Authors
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L.H.Weaver,
B.W.Matthews.
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Ref.
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j mol biol, 1987,
193,
189.
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