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UniProt functional annotation for P00614 | ||
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| UniProt code: P00614. |
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| Organism: | |
Oxyuranus scutellatus scutellatus (Australian taipan) (Coastal taipan). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera; Serpentes; Colubroidea; Elapidae; Acanthophiinae; Oxyuranus. |
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| Function: | |
Heterotrimer: Snake venom phospholipase A2 (PLA2) heterotrimer that acts as a potent presynaptic neurotoxin by blocking synaptic transmission and synaptic vesicle recycling. May act by binding in a calcium-dependent fashion to neurotonal pentraxin-1 (NPTX1) and neurotonal pentraxin-2 (NPTX2), but not to neuronal pentraxin receptor (NPTXR). Also binds to taipoxin-associated calcium binding protein 49 (RCN2), a protein localized in the lumen of endoplasmic reticulum. |
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| Function: | |
Monomer (alpha chain): Snake venom phospholipase A2 (PLA2) alpha chain that possesses the same high enzymatic activity as the heterotrimer. PLA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. |
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| Catalytic activity: | |
Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn- glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:15801, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:57643, ChEBI:CHEBI:58168; EC=3.1.1.4; Evidence={ECO:0000255|PROSITE-ProRule:PRU10035, ECO:0000255|PROSITE- ProRule:PRU10036, ECO:0000269|PubMed:22776098}; |
| Cofactor: | |
Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250}; Note=Binds 1 Ca(2+) ion. {ECO:0000250}; |
| Subunit: | |
Heterotrimer of alpha, beta, and gamma chains; non-covalently linked. {ECO:0000269|PubMed:976268}. |
| Subcellular location: | |
Secreted. |
| Tissue specificity: | |
Expressed by the venom gland. |
| Mass spectrometry: | |
Mass=13987.9; Mass_error=0.8; Method=Electrospray; Evidence={ECO:0000269|PubMed:22776098}; |
| Toxic dose: | |
Monomer (alpha chain): LD(50) is 0.3 mg/kg by intravenous injection into mice. {ECO:0000269|PubMed:976268}. |
| Toxic dose: | |
Heterotrimer: LD(50) is 2 ug/kg by intravenous injection into mice. {ECO:0000269|PubMed:976268}. |
| Similarity: | |
Belongs to the phospholipase A2 family. Group I subfamily. D49 sub-subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.