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UniProt functional annotation for P47228 | ||
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| UniProt code: P47228. |
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| Organism: | |
Paraburkholderia xenovorans (strain LB400). |
| Taxonomy: | |
Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia. |
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| Function: | |
Shows a preference for catechols with groups immediately adjacent to the hydroxyl substituents. |
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| Catalytic activity: | |
Reaction=biphenyl-2,3-diol + O2 = 2-hydroxy-6-oxo-6-phenylhexa-2,4- dienoate + H(+); Xref=Rhea:RHEA:14413, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16205, ChEBI:CHEBI:58284; EC=1.13.11.39; |
| Cofactor: | |
Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Note=Binds 1 Fe(2+) ion per subunit.; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=7 uM for biphenyl-2,3-diol; Note=Substrate inhibition occurs at 300 uM (+/- 20 uM).; pH dependence: Optimum pH is 8.0.; |
| Pathway: | |
Xenobiotic degradation; biphenyl degradation; 2-hydroxy-2,4- pentadienoate and benzoate from biphenyl: step 3/4. |
| Subunit: | |
Homooctamer. The enzyme is composed of two planar tetramers rotated at 45 degrees relative to each other, with a channel in the middle. {ECO:0000269|PubMed:8428946}. |
| Similarity: | |
Belongs to the extradiol ring-cleavage dioxygenase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.