UniProt functional annotation for P0A7Y4



	UniProt functional annotation for P0A7Y4

UniProt code: P0A7Y4.

Organism: Escherichia coli (strain K12).

Taxonomy: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.

Function: Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer.

Catalytic activity: Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.4;

Cofactor: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:9852071}; Note=Binds 1 Mg(2+) ion per subunit in the absence of substrate. Requires millimolar levels of Mg(2+) for maximal activity. Has low activity at micromolar concentrations of Mn(2+) and is inhibited at higher Mn(2+) levels. Can bind a second metal ion at a regulatory site, or after substrate binding. {ECO:0000269|PubMed:9852071};

Subunit: Monomer. {ECO:0000269|PubMed:11083878, ECO:0000269|PubMed:1311386, ECO:0000269|PubMed:15644213, ECO:0000269|PubMed:2169648}.

Subcellular location: Cytoplasm {ECO:0000305}.

Similarity: Belongs to the RNase H family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Escherichia coli (strain K12).
Taxonomy:		Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.



Function:		Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer.


Catalytic activity:		Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.4;
Cofactor:		Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269\|PubMed:9852071}; Note=Binds 1 Mg(2+) ion per subunit in the absence of substrate. Requires millimolar levels of Mg(2+) for maximal activity. Has low activity at micromolar concentrations of Mn(2+) and is inhibited at higher Mn(2+) levels. Can bind a second metal ion at a regulatory site, or after substrate binding. {ECO:0000269\|PubMed:9852071};
Subunit:		Monomer. {ECO:0000269\|PubMed:11083878, ECO:0000269\|PubMed:1311386, ECO:0000269\|PubMed:15644213, ECO:0000269\|PubMed:2169648}.
Subcellular location:		Cytoplasm {ECO:0000305}.
Similarity:		Belongs to the RNase H family. {ECO:0000305}.