|
|
||
|
|
|
|
|
UniProt functional annotation for P08164 | ||
|
|
|
|
|
|
||
| UniProt code: P08164. |
|
||
| Organism: | |
Bacillus subtilis (strain 168). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus. |
|
||
|
||
| Function: | |
Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source. {ECO:0000255|HAMAP- Rule:MF_00193, ECO:0000269|PubMed:7890752}. |
|
||
| Catalytic activity: | |
Reaction=ATP + deamido-NAD(+) + NH4(+) = AMP + diphosphate + H(+) + NAD(+); Xref=Rhea:RHEA:21188, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57540, ChEBI:CHEBI:58437, ChEBI:CHEBI:456215; EC=6.3.1.5; Evidence={ECO:0000255|HAMAP-Rule:MF_00193, ECO:0000269|PubMed:7890752}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.62 mM for NH(4)(+) {ECO:0000269|PubMed:7890752}; KM=0.28 mM for deamido-NAD {ECO:0000269|PubMed:7890752}; KM=0.21 mM for ATP {ECO:0000269|PubMed:7890752}; pH dependence: Optimum pH is 8.2-8.7. {ECO:0000269|PubMed:7890752}; Temperature dependence: Optimum temperature is 40-45 degrees Celsius. {ECO:0000269|PubMed:7890752}; |
| Pathway: | |
Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. {ECO:0000255|HAMAP- Rule:MF_00193, ECO:0000269|PubMed:7890752}. |
| Subunit: | |
Homodimer. {ECO:0000255|HAMAP-Rule:MF_00193, ECO:0000269|PubMed:11375500, ECO:0000269|PubMed:12077433, ECO:0000269|PubMed:7890752, ECO:0000269|PubMed:8895556, ECO:0000269|PubMed:9753692}. |
| Developmental stage: | |
Synthesis starts during germination and outgrowth, and is highest at the end of exponential growth. Present in dormant spores. {ECO:0000269|PubMed:7890752}. |
| Induction: | |
By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation. |
| Ptm: | |
Phosphorylated. {ECO:0000269|PubMed:1556067}. |
| Similarity: | |
Belongs to the NAD synthetase family. {ECO:0000255|HAMAP- Rule:MF_00193, ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.