 |
PDBsum entry 1koc
|
|
|
|
References listed in PDB file
|
 |
|
Key reference
|
|
|
Title
|
|
Structural basis of ligand discrimination by two related RNA aptamers resolved by nmr spectroscopy.
|
|
|
Authors
|
|
Y.Yang,
M.Kochoyan,
P.Burgstaller,
E.Westhof,
M.Famulok.
|
|
|
Ref.
|
|
Science, 1996,
272,
1343-1347.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
In a previous study, an RNA aptamer for the specific recognition of arginine was
evolved from a parent sequence that bound citrulline specifically. The two RNAs
differ at only 3 positions out of 44. The solution structures of the two
aptamers complexed to their cognate amino acids have now been determined by
two-dimensional nuclear magnetic resonance spectroscopy. Both aptamers contain
two asymmetrical internal loops that are not well ordered in the free RNA but
that fold into a compact structure upon ligand binding. Those nucleotides common
to both RNAs include a conserved cluster of purine residues, three of which form
an uneven plane containing a G:G pair, and two other residues nearly
perpendicular to that surface. Two of the three variant nucleotides are stacked
on the cluster of purines and form a triple contact to the amino acid side
chain, whereas the edge of the third variant nucleotide is capping the binding
pocket.
|
|
|
Secondary reference #1
|
|
|
Title
|
|
Structural probing and damage selection of citrulline- And arginine-Specific RNA aptamers identify base positions required for binding.
|
|
|
Authors
|
|
P.Burgstaller,
M.Kochoyan,
M.Famulok.
|
|
|
Ref.
|
|
Nucleic Acids Res, 1995,
23,
4769-4776.
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Headers
|
|
|
|
|
|
