PDBsum entry 1knw

Lyase

PDB id: 1knw

Contents

Protein chain

421 a.a. *

Ligands

SO4

PLP

MES

Metals

_LI ×2

Waters ×393

* Residue conservation analysis

PDB id:

1knw

Name:

Lyase

Title:

Crystal structure of diaminopimelate decarboxylase

Structure:

Diaminopimelate decarboxylase. Chain: a. Synonym: dap decarboxylase. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 562. Gene: lysa. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Biol. unit:

Dimer (from PDB file)

Resolution:

2.10Å R-factor: 0.173 R-free: 0.220

Authors:

V.Levdikov,L.Blagova,N.Bose,C.Momany

Key ref:

V.Levdikov et al. Diaminopimelate decarboxylase uses a versatile active site for stereospecific decarboxylation. To be published, .

Date:

19-Dec-01 Release date: 11-Nov-03

Protein chain

P00861  (DCDA_ECOLI) -  Diaminopimelate decarboxylase from Escherichia coli (strain K12)

Seq: 420 a.a.

Struc: 421 a.a.

Enzyme reactions

• Enzyme class: E.C.4.1.1.20 - diaminopimelate decarboxylase.
• Pathway: Lysine biosynthesis (later stages)
• Reaction: meso-2,6-diaminopimelate + H⁺ = L-lysine + CO2
• Cofactor: Pyridoxal 5'-phosphate

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = PLP) matches with 93.75% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site