PDBsum entry 1kn4

Immune system

PDB id: 1kn4

Contents

Protein chains

219 a.a. *

222 a.a. *

Ligands

PDE

Metals

_ZN ×7

Waters ×151

* Residue conservation analysis

References listed in PDB file

Key reference

• Title: Remarkable remote chiral recognition in a reaction mediated by a catalytic antibody.
• Authors: L.J.D'Souza, B.Gigant, M.Knossow, B.S.Green.
• Ref.: J Am Chem Soc, 2002, 124, 2114-2115. [DOI no: 10.1021/ja0170504]
• PubMed id: 11878955

Abstract

The crystal structures of catalytic antibody D2.3 Fab with the two enantiomers, 7D and 7L, which represent transition state analogues for the hydrolysis of the corresponding esters, 6D and 6L, were determined to better understand remarkable reactivity differences: the L-ester displayed significantly tighter binding (K(M)) and increased catalytic activity (k(cat)) with D2.3, even though the chiral center is 7 bonds distant from the reaction center. Surprisingly, the electron densities of the liganded phosphonates, 7D and 7L, within the D2.3 binding/reaction site were essentially identical, highlighting the subtle influences of protein interactions on chemical behavior.