 |
PDBsum entry 1kib
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Electron transport
|
PDB id
|
|
|
|
1kib
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
* Residue conservation analysis
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
Structure of cytochrome c6 from arthrospira maxima: an assembly of 24 subunits in a nearly symmetric shell.
|
|
|
Authors
|
|
C.A.Kerfeld,
M.R.Sawaya,
D.W.Krogmann,
T.O.Yeates.
|
|
|
Ref.
|
|
Acta Crystallogr D Biol Crystallogr, 2002,
58,
1104-1110.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
Cytochrome c(6) from the cyanobacterium Arthrospira maxima is present in
isoforms that can be resolved by size-exclusion chromatography. One isoform
crystallized in space group I4(1)32 with eight protein molecules in the
asymmetric unit and a total of 384 molecules in the unit cell. Within the
crystal, the molecules are arranged as clusters of 24 cytochrome c(6) molecules.
Each cluster is a hollow shell with approximate octahedral (432) symmetry.
Structural and biochemical studies of cytochrome c(6) isolated from other
cyanobacteria and algae have led to the suggestion that cytochrome c(6) forms
oligomers. The cytochrome c(6) complex described here is the largest assembly of
cytochrome c(6) molecules observed thus far.
|
|
|
|
|
|
|
|
Figure 2.
Figure 2 The A. maxima cytochrome c[6] 24-molecule cluster,
viewed down the (a) threefold and (b) pseudo-fourfold axis. In
(b) the side chains of Met19 which flank the pore are shown.
This figure and Figs. 3-and 5-(a) were drawn with RIBBONS
(Carson, 1997[Carson, M. (1997). Methods Enzymol. 277,
493-505.]).
|
|
Figure 5.
Figure 5 (a) Comparison of the A. maxima cytochrome c[6] trimer
to trimers observed in the crystal structures of C. reinhardtii
(Kerfeld et al., 1995[Kerfeld, C. A., Anwar, H. P., Interrante,
R., Merchant, S. & Yeates, T. O. (1995). J. Mol. Biol. 250,
627-647.]) and M. braunii (Frazao et al., 1995[Frazao, C.,
Soares, C. M., Carrondo, M. A., Pohl, E., Dauter, Z., Wilson, K.
S., Hervas, M., Navarro, J. A., De la Rosa, M. A. & Sheldrick,
G. M. (1995). Structure, 3, 1159-1169.]) cytochrome c[6]. (b)
and (c) Comparison of the electrostatic surface potential of the
trimers. The view shown in (b) is the same as in (a); the view
shown in (c) shows the opposite face.
|
|
|
|
|
|
The above figures are
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(2002,
58,
1104-1110)
copyright 2002.
|
|
|
Secondary reference #1
|
|
|
Title
|
|
Structures of cytochrome c-549 and cytochrome c6 from the cyanobacterium arthrospira maxima.
|
|
|
Authors
|
|
M.R.Sawaya,
D.W.Krogmann,
A.Serag,
K.K.Ho,
T.O.Yeates,
C.A.Kerfeld.
|
|
|
Ref.
|
|
Biochemistry, 2001,
40,
9215-9225.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
|
|
|
|
