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UniProt functional annotation for P0AC47 | ||
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| UniProt codes: P0AC47, P00364. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; fumarate reductase is used during anaerobic growth, and succinate dehydrogenase is used during aerobic growth. The QFR enzyme complex binds 2 quinones in or near the membrane; 1 near the [3Fe-4S] cluster (QP is proximal to the [3Fe-4S] cluster, on the cytoplasmic side of the membrane) while QD (the distal cluster) is on the other side of the membrane. It is not clear if both of the quinol- binding sites are functionally relevant (PubMed:10373108, PubMed:11850430). {ECO:0000269|PubMed:10373108, ECO:0000269|PubMed:11850430}. |
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| Catalytic activity: | |
Reaction=a quinone + succinate = a quinol + fumarate; Xref=Rhea:RHEA:40523, ChEBI:CHEBI:24646, ChEBI:CHEBI:29806, ChEBI:CHEBI:30031, ChEBI:CHEBI:132124; EC=1.3.5.4; Evidence={ECO:0000269|PubMed:11850430}; |
| Catalytic activity: | |
Reaction=a menaquinone + succinate = a menaquinol + fumarate; Xref=Rhea:RHEA:27834, Rhea:RHEA-COMP:9537, Rhea:RHEA-COMP:9539, ChEBI:CHEBI:16374, ChEBI:CHEBI:18151, ChEBI:CHEBI:29806, ChEBI:CHEBI:30031; EC=1.3.5.4; Evidence={ECO:0000305|PubMed:10373108}; |
| Cofactor: | |
Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:49601; Evidence={ECO:0000269|PubMed:10373108}; Note=Binds 1 [2Fe-2S] cluster. {ECO:0000269|PubMed:10373108}; |
| Cofactor: | |
Name=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137; Evidence={ECO:0000269|PubMed:10373108}; Note=Binds 1 [3Fe-4S] cluster. {ECO:0000269|PubMed:10373108}; |
| Cofactor: | |
Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000269|PubMed:10373108}; Note=Binds 1 [4Fe-4S] cluster. {ECO:0000269|PubMed:10373108}; |
| Subunit: | |
Part of an enzyme complex containing four subunits: a flavoprotein (FrdA), an iron-sulfur protein (FrdB), and two hydrophobic anchor proteins (FrdC and FrdD). {ECO:0000269|PubMed:10373108, ECO:0000269|PubMed:11850430}. |
| Subcellular location: | |
Cell inner membrane {ECO:0000305|PubMed:10373108}; Peripheral membrane protein {ECO:0000305|PubMed:10373108}; Cytoplasmic side {ECO:0000305|PubMed:10373108}. |
| Similarity: | |
Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.