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UniProt functional annotation for P25984 | ||
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| UniProt code: P25984. |
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| Organism: | |
Clostridium beijerinckii (Clostridium MP). |
| Taxonomy: | |
Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium. |
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| Function: | |
Alcohol dehydrogenase with a preference for medium chain secondary alcohols, such as 2-butanol and isopropanol. Has very low activity with primary alcohols, such as ethanol. Under physiological conditions, the enzyme reduces aldehydes and 2-ketones to produce secondary alcohols. Is active with acetaldehyde and propionaldehyde. {ECO:0000269|PubMed:20102159, ECO:0000269|PubMed:8349550}. |
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| Catalytic activity: | |
Reaction=NADP(+) + propan-2-ol = acetone + H(+) + NADPH; Xref=Rhea:RHEA:21792, ChEBI:CHEBI:15347, ChEBI:CHEBI:15378, ChEBI:CHEBI:17824, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.80; Evidence={ECO:0000269|PubMed:20102159, ECO:0000269|PubMed:8349550}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:20102159, ECO:0000269|PubMed:8349550, ECO:0000269|PubMed:9836873}; Note=Binds 1 zinc ion per subunit. {ECO:0000269|PubMed:20102159, ECO:0000269|PubMed:8349550, ECO:0000269|PubMed:9836873}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=5.7 mM for 2-butanol {ECO:0000269|PubMed:8349550}; KM=10 mM for isopropanol {ECO:0000269|PubMed:8349550}; KM=5.2 mM for 2-pentanol {ECO:0000269|PubMed:8349550}; KM=1 mM for acetone {ECO:0000269|PubMed:8349550}; KM=1.5 mM for 2-butanone {ECO:0000269|PubMed:8349550}; KM=0.03 mM for NADPH {ECO:0000269|PubMed:8349550}; pH dependence: Optimum pH is 8. {ECO:0000269|PubMed:8349550}; |
| Subunit: | |
Homotetramer. {ECO:0000269|PubMed:12381840, ECO:0000269|PubMed:20102159, ECO:0000269|PubMed:8349550, ECO:0000269|PubMed:9836873}. |
| Similarity: | |
Belongs to the zinc-containing alcohol dehydrogenase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.