 |
PDBsum entry 1kb5
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Complex (immunoglobulin/receptor)
|
PDB id
|
|
|
|
1kb5
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
|
115 a.a.
|
|
|
|
|
|
|
|
|
|
|
117 a.a.
|
|
|
|
|
|
|
|
|
|
|
214 a.a.
|
|
|
|
|
|
|
|
|
|
|
219 a.a.
|
|
|
|
|
|
|
|
|
* Residue conservation analysis
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
The three-Dimensional structure of a t-Cell antigen receptor V alpha V beta heterodimer reveals a novel arrangement of the v beta domain.
|
|
|
Authors
|
|
D.Housset,
G.Mazza,
C.Grégoire,
C.Piras,
B.Malissen,
J.C.Fontecilla-Camps.
|
|
|
Ref.
|
|
EMBO J, 1997,
16,
4205-4216.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
The crystal structure of a mouse T-cell antigen receptor (TCR) Fv fragment
complexed to the Fab fragment of a specific anti-clonotypic antibody has been
determined to 2.6 A resolution. The polypeptide backbone of the TCR V alpha
domain is very similar to those of other crystallographically determined V
alphas, whereas the V beta structure is so far unique among TCR V beta domains
in that it displays a switch of the c" strand from the inner to the outer
beta-sheet. The beta chain variable region of this TCR antigen-binding site is
characterized by a rather elongated third complementarity-determining region
(CDR3beta) that packs tightly against the CDR3 loop of the alpha chain, without
leaving any intervening hydrophobic pocket. Thus, the conformation of the CDR
loops with the highest potential diversity distinguishes the structure of this
TCR antigen-binding site from those for which crystallographic data are
available. On the basis of all these results, we infer that a significant
conformational change of the CDR3beta loop found in our TCR is required for
binding to its cognate peptide-MHC ligand.
|
|
|
|
|
|
|
|
Figure 3.
Figure 3 Stereoscopic view of the KB5-C20 V  -V
 interface.
The V and
V domains
are on the left and right sides, respectively. Only side chains
of residues involved in interdomain interactions are shown.
Residues are colour-coded according to their chemical nature.
Acidic residues are red, basic residues dark blue, polar
residues light blue, hydrophobic residues yellow and aromatic
residues purple.
|
|
Figure 7.
Figure 7 Stereo pairs of the electron density map of the model
contoured at the  level,
and generated from SIGMAA-weighted 2mF[o]-DF[c] coefficients
(Read, 1986). (A) The switched c" strand and the d strand of the
V domain
are shown to illustrate their extended interactions. (B) The
CDR3 loop
is very well defined in the electron density map.
|
|
|
|
|
|
The above figures are
reprinted
from an Open Access publication published by Macmillan Publishers Ltd:
EMBO J
(1997,
16,
4205-4216)
copyright 1997.
|
|
|
Secondary reference #1
|
|
|
Title
|
|
An alphabeta t cell receptor structure at 2.5 a and its orientation in the tcr-Mhc complex.
|
|
|
Authors
|
|
K.C.Garcia,
M.Degano,
R.L.Stanfield,
A.Brunmark,
M.R.Jackson,
P.A.Peterson,
L.Teyton,
I.A.Wilson.
|
|
|
Ref.
|
|
Science, 1996,
274,
209-219.
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
Secondary reference #2
|
|
|
Title
|
|
Structure of the complex between human t-Cell receptor, Viral peptide and hla-A2.
|
|
|
Authors
|
|
D.N.Garboczi,
P.Ghosh,
U.Utz,
Q.R.Fan,
W.E.Biddison,
D.C.Wiley.
|
|
|
Ref.
|
|
Nature, 1996,
384,
134-141.
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
Secondary reference #3
|
|
|
Title
|
|
Crystal structure of the beta chain of a t cell antigen receptor.
|
|
|
Authors
|
|
G.A.Bentley,
G.Boulot,
K.Karjalainen,
R.A.Mariuzza.
|
|
|
Ref.
|
|
Science, 1995,
267,
1984-1987.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
Secondary reference #4
|
|
|
Title
|
|
Crystal structure of the V alpha domain of a t cell antigen receptor.
|
|
|
Authors
|
|
B.A.Fields,
B.Ober,
E.L.Malchiodi,
M.I.Lebedeva,
B.C.Braden,
X.Ysern,
J.K.Kim,
X.Shao,
E.S.Ward,
R.A.Mariuzza.
|
|
|
Ref.
|
|
Science, 1995,
270,
1821-1824.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
Secondary reference #5
|
|
|
Title
|
|
Engineered secreted t-Cell receptor alpha beta heterodimers.
|
|
|
Authors
|
|
C.Grégoire,
N.Rebaï,
F.Schweisguth,
A.Necker,
G.Mazza,
N.Auphan,
A.Millward,
A.M.Schmitt-Verhulst,
B.Malissen.
|
|
|
Ref.
|
|
Proc Natl Acad Sci U S A, 1991,
88,
8077-8081.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
|
|
|
|
