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UniProt functional annotation for O66640 | ||
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| UniProt code: O66640. |
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| Organism: | |
Aquifex aeolicus (strain VF5). |
| Taxonomy: | |
Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex. |
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| Function: | |
Required for rescue of stalled ribosomes mediated by trans- translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans- translation. {ECO:0000255|HAMAP-Rule:MF_00023}. |
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| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00023}. Note=The tmRNA-SmpB complex associates with stalled 70S ribosomes. {ECO:0000255|HAMAP-Rule:MF_00023}. |
| Miscellaneous: | |
Although the Valle et al., electron microscopy paper indicates this protein came from T.thermophilus its sequence maps to A.aeolicus. {ECO:0000305|PubMed:12677067}. |
| Similarity: | |
Belongs to the SmpB family. {ECO:0000255|HAMAP- Rule:MF_00023}. |
Annotations taken from UniProtKB at the EBI.