PDBsum entry 1k8b

Translation

PDB id

1k8b

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Contents

			Protein chain

					52 a.a. *

* Residue conservation analysis

PDB id:

1k8b

Links

Name:

Translation

Title:

Nmr structure analysis of the n-terminal domain of archaeal translation initiation factor 2 subunit beta

Structure:

Probable translation initiation factor 2 beta subunit. Chain: a. Synonym: eif-2-beta. Engineered: yes

Source:

Methanocaldococcus jannaschii. Organism_taxid: 2190. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

NMR struc:

10 models

Authors:

S.Cho,D.W.Hoffman

Key ref:

S.Cho and D.W.Hoffman (2002). Structure of the beta subunit of translation initiation factor 2 from the archaeon Methanococcus jannaschii: a representative of the eIF2beta/eIF5 family of proteins. Biochemistry, 41, 5730-5742. PubMed id: 11980477 DOI: 10.1021/bi011984n

Date:

23-Oct-01

Release date:

24-Apr-02

PROCHECK

	Headers

	References

Protein chain

Q57562 (IF2B_METJA) - Translation initiation factor 2 subunit beta from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

Seq: Struc:					143 a.a. 52 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.?

[IntEnz] [ExPASy] [KEGG] [BRENDA]

DOI no: 10.1021/bi011984n	Biochemistry 41:5730-5742 (2002)
PubMed id: 11980477

Structure of the beta subunit of translation initiation factor 2 from the archaeon Methanococcus jannaschii: a representative of the eIF2beta/eIF5 family of proteins.
S.Cho, D.W.Hoffman.

ABSTRACT

The beta subunit of archaeal translation initiation factor 2 (aIF2beta) is a representative of a family of proteins whose members include the beta subunit of eukaryotic translation initiation factor 2 (eIF2beta) and the N-terminal domain within translation initiation factor 5 (eIF5); no members of this family of proteins have been structurally characterized up to this time. In the work presented here, aIF2beta from Methanococcus jannaschii was expressed in Escherichia coli, purified, and analyzed using multidimensional NMR methods. The aIF2beta was found to contain two independent structural domains. The N-terminal domain contains a four-stranded antiparallel beta sheet and two alpha helices, and is structurally similar to the DNA-binding domain of a yeast heat shock transcription factor and a domain within ribosomal protein S4. This structural similarity was an unanticipated result, since no significant homology was detected at the level of primary sequence. The C-terminal domain of aIF2beta contains a zinc-binding motif of three antiparallel beta strands, with four conserved cysteines arranged as two CXXC units separated by 17 residues. Conserved residues on the surface of each domain that are likely candidates for direct interaction with other components of the translational apparatus were identified. The significant primary sequence homology between archaeal aIF2beta and the eukaryotic eIF2beta and eIF5, when combined with the structural results in the work presented here, permitted structural features to be predicted for these latter two eukaryotic proteins.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21568863

E.A.Stolboushkina, and M.B.Garber (2011).
Eukaryotic type translation initiation factor 2: structure-functional aspects.

Biochemistry (Mosc), 76, 283-294.

19703275

A.Y.Mulkidjanian, and M.Y.Galperin (2009).
On the origin of life in the Zinc world. 2. Validation of the hypothesis on the photosynthesizing zinc sulfide edifices as cradles of life on Earth.

Biol Direct, 4, 27.

19548767

T.Bereau, and M.Deserno (2009).
Generic coarse-grained model for protein folding and aggregation.

J Chem Phys, 130, 235106.

18000047

L.Yatime, Y.Mechulam, S.Blanquet, and E.Schmitt (2007).
Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states.

Proc Natl Acad Sci U S A, 104, 18445-18450.

PDB codes:

2qmu 2qn6

16924118

M.Sokabe, M.Yao, N.Sakai, S.Toya, and I.Tanaka (2006).
Structure of archaeal translational initiation factor 2 betagamma-GDP reveals significant conformational change of the beta-subunit and switch 1 region.

Proc Natl Acad Sci U S A, 103, 13016-13021.

PDB codes:

2d74 2dcu

14978306

P.Gutiérrez, M.J.Osborne, N.Siddiqui, J.F.Trempe, C.Arrowsmith, and K.Gehring (2004).
Structure of the archaeal translation initiation factor aIF2 beta from Methanobacterium thermoautotrophicum: implications for translation initiation.

Protein Sci, 13, 659-667.

PDB code:

1nee

12581660

N.Sonenberg, and T.E.Dever (2003).
Eukaryotic translation initiation factors and regulators.

Curr Opin Struct Biol, 13, 56-63.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.